O'Reilly M, Silver G M, Gamelli R L, Davis J H, Hebert J C
Department of Anesthesia, University of Vermont College of Medicine, Burlington.
J Trauma. 1994 Apr;36(4):486-90. doi: 10.1097/00005373-199404000-00004.
Infections remain a serious problem following injury. Immune modulation offers an additional strategy for the treatment of infections. We evaluated the ability of a multilineage hematopoietic growth factor, granulocyte-macrophage colony-stimulating factor (GM-CSF), to improve survival following burn injury with a superimposed burn wound infection. Groups of 12 BDF1 mice received a 15% total body surface area (TBSA) thermal injury by immersion in 100 degrees C water; 6 x 10(3) Pseudomonas was then applied to the burn wound. The GM-CSF was injected subcutaneously B.I.D. for 7 days. Mice receiving the 10-ng dose of GM-CSF had significantly improved survival compared with the controls; other doses had no significant effect on survival. Clinical trials to assess the ability of GM-CSF to reduce infectious complications following burn injury are underway and these data suggest selecting a specific dose may be critical in achieving maximal benefit.
受伤后感染仍然是一个严重的问题。免疫调节为感染的治疗提供了一种额外的策略。我们评估了一种多谱系造血生长因子，即粒细胞巨噬细胞集落刺激因子（GM-CSF），在伴有叠加性烧伤创面感染的烧伤后提高存活率的能力。将12只BDF1小鼠分为一组，通过浸入100摄氏度的水中造成15%体表面积（TBSA）的热损伤；然后将6×10³ 铜绿假单胞菌应用于烧伤创面。GM-CSF以每日两次的频率皮下注射，持续7天。与对照组相比，接受10纳克剂量GM-CSF的小鼠存活率显著提高；其他剂量对存活率没有显著影响。评估GM-CSF降低烧伤后感染并发症能力的临床试验正在进行中，这些数据表明选择特定剂量对于实现最大益处可能至关重要。
