Gaydos J M, Carrick L, Berk R S
Proc Soc Exp Biol Med. 1976 Mar;151(3):603-7. doi: 10.3181/00379727-151-39269.
Mice were found to be generally refractory to sc challenge with heat-killed cells of Pseudomonas aeruginosa and did not die unless unusually high concentrations were employed. Approximately 38.6% of the animals receiving a single, sublethal dose of 1 X 10(10) dead cells developed black, crusty, necrotic skin lesions within 3 to 5 days. No major gross and histopathological changes were detected in internal organs. If the animals were sc administered sublethal doses of either live or dead cells of P aeruginosa 16 days prior to sc challenge, then the incidence of black lesions rose to 78.6 and 50% of the animals, respectively. Of several antineoplastic agents tested, only methotrexate significantly affected the 72-hr LD50 resulting in a drop to 1.8 X 10(9) afrom 3.4 X 10(10) cells. However, both methotrexate and actinomycin D decreased the incidence of the black lesions.
研究发现，小鼠对皮下注射热灭活的铜绿假单胞菌细胞通常具有抗性，除非使用异常高的浓度，否则不会死亡。接受单次亚致死剂量1×10¹⁰个死细胞的动物中，约38.6%在3至5天内出现黑色、结痂、坏死的皮肤病变。在内脏器官中未检测到重大的大体和组织病理学变化。如果在皮下注射挑战前16天给动物皮下注射亚致死剂量的活的或死的铜绿假单胞菌细胞，那么黑色病变的发生率分别上升到78.6%和50%。在测试的几种抗肿瘤药物中，只有甲氨蝶呤显著影响72小时半数致死剂量，导致其从3.4×10¹⁰个细胞降至1.8×10⁹个细胞。然而，甲氨蝶呤和放线菌素D都降低了黑色病变的发生率。
