Beck F W, Whitehouse M W, Pearson C M
Proc Soc Exp Biol Med. 1976 Mar;151(3):615-22. doi: 10.3181/00379727-151-39272.
Several methods of inducing experimental allergic encephalomyelitis (EAE) in rats were examined using different (i) rat strains, (ii) combinations of encephalitogen with different adjuvants, and (iii) sites of encephalitogen inoculation. The time course and severity of the ensuing diseases were determined and methods delineated for inducing a disease with limited variability and high incidence. Omitting the mycobacterial component from the adjuvant eliminated the complication of adjuvant arthritis, which may develop after the appearance of EAE. Encephalitogenic emulsions prepared with an equal volume of frozen guinea pig spinal cord (GPSC) and hexadecane or squalene, injected into two inguinal nodes or one foot pad of Lewis rats, provided two quick and easy ways to induce EAE. Emulsions of encephalitogen with Freund's complete adjuvant or hexadecane, injected into the ear, also induced EAE but lengthened the time between the antigen inoculation and clinical symptoms which accompany the onset of EAE disease. However, injection into the ear offers an advantage over the Newbould technique (direct instillation of encaphalitogen in pre-exposed lymph nodes), since the animals can be confidently predosed with drugs which may reduce lymphoid mass. Effects of local inflammation on systemic drug metabolism are also minimized when using the ear route.
使用不同的（i）大鼠品系、（ii）脑脊髓炎原与不同佐剂的组合以及（iii）脑脊髓炎原接种部位，研究了几种在大鼠中诱导实验性变应性脑脊髓炎（EAE）的方法。确定了随后疾病的时间进程和严重程度，并描述了诱导变异性有限且发病率高的疾病的方法。从佐剂中省略分枝杆菌成分可消除佐剂性关节炎的并发症，佐剂性关节炎可能在EAE出现后发生。用等体积的冷冻豚鼠脊髓（GPSC）和十六烷或角鲨烷制备的致脑脊髓炎乳剂，注入Lewis大鼠的两个腹股沟淋巴结或一个足垫，提供了两种快速简便的诱导EAE的方法。将脑脊髓炎原与弗氏完全佐剂或十六烷制成的乳剂注入耳部，也可诱导EAE，但延长了抗原接种与EAE疾病发作伴随的临床症状之间的时间。然而，与纽博尔德技术（将脑脊髓炎原直接滴入预先暴露的淋巴结）相比，耳部注射具有优势，因为可以放心地给动物预先服用可能减少淋巴组织量的药物。使用耳部途径时，局部炎症对全身药物代谢的影响也会降至最低。
