Robert L S, Allard S, Gerster J L, Cass L, Simmonds J
Plant Research Centre, Central Experimental Farm, Ottawa, Ontario, Canada.
Plant Mol Biol. 1994 Nov;26(4):1217-22. doi: 10.1007/BF00040703.
A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these transcripts are expressed in the stigma throughout flower development. Pis 63-1 and Pis 63-2 display similarity to a cotton fibre cDNA clone.
通过差异筛选分离出一个部分cDNA克隆Pis 63，它对应于在甘蓝型油菜雌蕊中高表达的一种mRNA。利用聚合酶链式反应（PCR）完成了Pis 63序列（Pis 63-1），并获得了另一个相关cDNA（Pis 63-2）的序列。Northern杂交和原位分析表明，这些转录本在整个花发育过程中都在柱头中表达。Pis 63-1和Pis 63-2与一个棉花纤维cDNA克隆具有相似性。
