Caccamo D V, Keohane M E, McKeever P E
Department of Pathology, Henry Ford Hospital, Detroit, Michigan.
Mod Pathol. 1994 Jan;7(1):99-104.
The tissue (tPA) and urokinase (uPA) types of plasminogen activator and the plasminogen activator inhibitor 1 (PAI-1) are enzymatic proteins that may play an important role in the degradation of the extracellular matrix in physiologic and neoplastic conditions. In particular, urokinase may underlie key properties of malignant cells, such as invasiveness and dissemination. We have studied the immunohistochemical distribution of tPA, uPA, and PAI-1 in 24 human gliomas, including seven well-differentiated astrocytomas, three oligodendrogliomas, six anaplastic astrocytomas, and eight glioblastomas multiforme. All anaplastic astrocytomas and glioblastomas showed numerous neoplastic cells immunoreactive for uPA, but not for tPA. In contrast, low-grade gliomas were negative for uPA, but contained some tPA-immunoreactive cells. Endothelial cells of vessels in non-neoplastic and neoplastic brain were immunoreactive for tPA, but not for uPA or PAI-1. Non-neoplastic glia were unreactive for tPA, uPA, and PAI-1. Small anaplastic cells present in three glioblastomas showed immunoreactivity for PAI-1. The presence of a large number of uPA-immunoreactive neoplastic cells in high-grade gliomas suggest that this fibrinolytic protein plays a significant role in the invasive properties of these neoplasms.
组织型纤溶酶原激活物（tPA）和尿激酶型纤溶酶原激活物（uPA）以及纤溶酶原激活物抑制剂1（PAI-1）是酶蛋白，在生理和肿瘤状态下的细胞外基质降解中可能起重要作用。特别是，尿激酶可能是恶性细胞关键特性的基础，如侵袭性和扩散。我们研究了tPA、uPA和PAI-1在24例人类胶质瘤中的免疫组化分布，包括7例高分化星形细胞瘤、3例少突胶质细胞瘤、6例间变性星形细胞瘤和8例多形性胶质母细胞瘤。所有间变性星形细胞瘤和胶质母细胞瘤均显示大量对uPA免疫反应阳性的肿瘤细胞，但对tPA免疫反应阴性。相比之下，低级别胶质瘤对uPA呈阴性，但含有一些对tPA免疫反应阳性的细胞。非肿瘤性和肿瘤性脑内血管的内皮细胞对tPA免疫反应阳性，但对uPA或PAI-1免疫反应阴性。非肿瘤性神经胶质细胞对tPA、uPA和PAI-1无反应。3例胶质母细胞瘤中存在的小间变性细胞对PAI-1免疫反应阳性。高级别胶质瘤中大量对uPA免疫反应阳性的肿瘤细胞的存在表明，这种纤维蛋白溶解蛋白在这些肿瘤的侵袭特性中起重要作用。
