Hassid A, Konieczkowski M, Dunn M J
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1155-9. doi: 10.1073/pnas.76.3.1155.
Isolated rat kidney glomeruli converted octatritiated arachidonic acid to several prostaglandins whose production was inhibited by meclofenamate. These were, in order of decreasing abundance, prostaglandin F2 alpha, prostaglandin E2, 6-oxo-prostaglandin F1 alpha, thromboxane B2, and prostaglandin D2. These products were identified by thin-layer chromatography, before and after treatment with potassium hydroxide or sodium borohydride. Prostaglandins F2 alpha and E2 were also determined by radioimmunoassay. The major product made by glomeruli was an unidentified substance(s), whose appearance was partially inhibited by meclofenamate, and was likely to be a hydroxylated fatty acid(s). The specific activity of glomerular fatty acid cyclo-oxygenase (EC 1.14.99.1), based on radioimmunoassay for prostaglandins E2 and F2 alpha, was 10- to 40-fold higher than that of cortical tubular enzyme. These data demonstrate that glomeruli have the capability of synthesizing an array of end-products from arachidonic acid. These prostaglandins may exert important physiologic effects, because renin secretion and arteriolar resistance are regulated by the glomerulus and the afferent and efferent arterioles.
分离的大鼠肾肾小球可将八氚化花生四烯酸转化为几种前列腺素，其生成受到甲氯芬那酸的抑制。这些前列腺素按丰度递减顺序依次为前列腺素F2α、前列腺素E2、6-氧代前列腺素F1α、血栓素B2和前列腺素D2。在用氢氧化钾或硼氢化钠处理前后，通过薄层色谱法对这些产物进行了鉴定。前列腺素F2α和E2也通过放射免疫测定法进行了测定。肾小球产生的主要产物是一种未鉴定的物质，其出现受到甲氯芬那酸的部分抑制，可能是一种羟基化脂肪酸。基于前列腺素E2和F2α的放射免疫测定，肾小球脂肪酸环氧化酶（EC 1.14.99.1）的比活性比皮质肾小管酶高10至40倍。这些数据表明，肾小球具有从花生四烯酸合成一系列终产物的能力。这些前列腺素可能发挥重要的生理作用，因为肾小球以及入球小动脉和出球小动脉可调节肾素分泌和小动脉阻力。
