Studies on the morphology of colonies of bacilli of BCG-Poland substrain. II. Dissociation of BCG-Poland substrain on Löwenstein-Jensen egg medium.

The dissociation of colonies of the substrain BCG-Poland on Löwenstein-Jensen medium has been studied. It had been stated previously (Lipińska and Rzucidło, 1974) that BCG-Poland substrain undergoes a process of dissociation which manifests itself in the appearance of smooth colonies alongside typical rough colonies. Attempts have been made to obtain pure culture of both colony types. Pure cultures composed of smooth or rough colonies respectively were obtained. The morphology and diameter of the smooth colonies depended on the size of the seed inoculum spread on the medium.