Extracellular dopamine levels within the striatum increase during inhalation exposure to toluene: a microdialysis study in awake, freely moving rats.

An exposure chamber for microdialysis on awake, freely moving rats during exposure to volatile agents is described. Inhalation exposure to 1000 and 2000 ppm toluene for 2 h was accompanied by an increase in extracellular dopamine levels within the striatum, but did not affect the homovanillic acid level. Neither the dopamine nor the homovanillic acid level was affected by toluene 500 ppm or isoamylacetate. It is suggested that the action of inhaled toluene on the dopamine neuron differs from that of the anaesthetic halothane, possibly by interfering with dopamine reuptake. Microdialysis seems to be a useful tool for studying the effects of volatile agents on brain neurotransmission.