采用高效液相色谱法同时测定人血浆中氨己烯酸、扑米酮、苯巴比妥、卡马西平、两种卡马西平代谢物、苯妥英和一种苯妥英代谢物的含量。

Simultaneous determination of felbamate, primidone, phenobarbital, carbamazepine, two carbamazepine metabolites, phenytoin, and one phenytoin metabolite in human plasma by high-performance liquid chromatography.

作者信息

Romanyshyn L A, Wichmann J K, Kucharczyk N, Shumaker R C, Ward D, Sofia R D

机构信息

Department of Biochemistry, Wallace Laboratories, Cranbury, New Jersey 08512.

出版信息

Ther Drug Monit. 1994 Feb;16(1):90-9. doi: 10.1097/00007691-199402000-00015.

DOI:10.1097/00007691-199402000-00015

PMID:8160262

Abstract

An isocratic liquid-chromatographic method employing one extraction step has been developed for the quantitation of five drugs and three metabolites in human plasma. The method uses 0.100-ml aliquots of human plasma and two internal standards. Chromatographic conditions include a 4.6 mm x 150 mm Spherisorb ODS2, 3 microns a high-performance liquid chromatography, (HPLC) column, a phosphate buffer-acetonitrile-methanol (700:160:140) mobile phase, and ultraviolet (UV) absorbance detection at 210 nm. Analytes and linear quantitation ranges (microgram/ml) were felbamate (FBM) 0.391-200; primidone (PRIM), 0.098-100; phenobarbital (PHENO), 0.195-100; carbamazepine (CBZ), 0.195-100; phenytoin (PHT), 0.195-200. For CBZ-transdiol (CBZ-TR) CBZ-epoxide (CBZ-EP), and the PHT metabolite, 5-(4-hydroxyphenyl)-5-phenylhydantoin (HPPH), the range was 0.049-25.0 micrograms/ml. Ethosuximide, methsuximide, 2-methyl-2-phenyl-succinimide (methsuximide metabolite), 2-ethyl-2-phenyl malonamide (PRIM metabolite, 5-ethyl-5-(4-hydroxyphenyl)-barbituric acid (PHENO metabolite), and mephenytoin do not interfere with quantitation of the above compounds.

摘要

已开发出一种采用单步萃取的等度液相色谱法，用于定量测定人血浆中的五种药物和三种代谢物。该方法使用0.100毫升人血浆等分试样和两种内标。色谱条件包括一根4.6毫米×150毫米、粒径为3微米的Spherisorb ODS2高效液相色谱（HPLC）柱、磷酸盐缓冲液-乙腈-甲醇（700:160:140）流动相以及在210纳米处的紫外（UV）吸光度检测。分析物及其线性定量范围（微克/毫升）分别为非氨酯（FBM）0.391 - 200；扑米酮（PRIM），0.098 - 100；苯巴比妥（PHENO），0.195 - 100；卡马西平（CBZ），0.195 - 100；苯妥英（PHT），0.195 - 200。对于卡马西平-反式二醇（CBZ-TR）、卡马西平-环氧化物（CBZ-EP）以及苯妥英代谢物5-(4-羟基苯基)-5-苯基乙内酰脲（HPPH），范围是0.049 - 25.0微克/毫升。乙琥胺、甲琥胺、2-甲基-2-苯基琥珀酰亚胺（甲琥胺代谢物）、2-乙基-2-苯基丙二酰胺（扑米酮代谢物）、5-乙基-5-(4-羟基苯基)-巴比妥酸（苯巴比妥代谢物）以及美芬妥英不干扰上述化合物的定量测定。