Stabler J G, McCormick T W, Powell K C, Kogut M H
Department of Biological Sciences, University of Arkansas, Fayetteville.
Vet Microbiol. 1994 Feb;38(4):293-305. doi: 10.1016/0378-1135(94)90148-1.
The ability of heterophils and monocytes from domestic chickens and turkeys to phagocytize and kill Salmonella enteritidis (SE) was investigated using both a standard plate count (chickens only) and direct microscopic examination by staining with acridine orange (both chickens and turkeys). Optimal phagocytosis of SE by both chicken and turkey phagocytes required opsonization of the bacteria by antibodies as determined by both assays. However, heterophils from both the turkeys and chickens phagocytized more SE than did monocytes from either type of fowl. Regardless of the presence or absence of antibodies, heterophils were more efficient bactericidal cells than were the monocytes since only heterophils killed the intracellular bacteria while the majority of nonopsonized SE survived in the monocytes. Phagocytosis of SE did not result in an increase in the amount of superoxide anion released by the heterophils suggesting that the ultimate fate of the bacteria in these cells was not related to an oxygen-dependent mechanism.
利用标准平板计数法（仅用于鸡）和吖啶橙染色直接显微镜检查法（用于鸡和火鸡），研究了家鸡和火鸡的异嗜性粒细胞和单核细胞吞噬和杀灭肠炎沙门氏菌（SE）的能力。两种检测方法均表明，鸡和火鸡的吞噬细胞对SE的最佳吞噬作用需要抗体对细菌进行调理。然而，火鸡和鸡的异嗜性粒细胞吞噬的SE比两种禽类的单核细胞都多。无论有无抗体，异嗜性粒细胞都是比单核细胞更有效的杀菌细胞，因为只有异嗜性粒细胞能杀死细胞内的细菌，而大多数未调理的SE在单核细胞中存活。SE的吞噬作用并未导致异嗜性粒细胞释放的超氧阴离子量增加，这表明这些细胞中细菌的最终命运与氧依赖性机制无关。
