Fujikura Y, Kuniki H, Sawada T, Hamano K, Akino T, Shigetomi M, Hirozane A, Fukumoto T
Department of Anatomy, Yamaguchi University School of Medicine, Japan.
Cell Transplant. 1994 Jan-Feb;3(1):23-31. doi: 10.1177/096368979400300105.
The expression of membrane antigens on rat hepatocytes transplanted syngeneically and heterotopically was analyzed immunohistochemically using monoclonal antibodies against rat hepatocytes. Isolated adult and fetal hepatocytes were able to survive in the spleen, salivary gland, thymus, or subcapsular region of the kidney for various periods after transplantation. Fairly clear expression of HAM2, 4, and 8 antigens was observed on hepatocytes transplanted into syngeneic spleen, suggesting that the cells might be functionally equivalent to hepatocytes in situ. HAM4 antigen was localized specifically on the newly formed bile-canalicular faces of hepatocytes. The expression of HAM2 (MHC class I) antigen on the transplanted hepatocytes appeared much stronger on the side facing lymphoid tissues, than on the other faces, suggesting that some immunological reactions may take place between hepatocytes and lymphoid tissue. HAM8 antigen, which is localized on gap junctions between neighboring hepatocytes in rat liver, was also recognized between transplanted hepatocytes. In salivary glands where hepatocytes were transplanted, bile-canaliculus-like structures were observed not only between neighboring hepatocytes but also between hepatocytes and salivary acinar cells, suggesting good interaction between the two different epithelial cell types. Hepatocytes transplanted into thymus appeared viable, but most showed fatty degeneration. Some healthy hepatocytes survived in the interlobular connective tissue and the thymic cortical tissue. When fetal hepatocytes were transplanted heterotopically, they formed a mass consisting of hepatocytes and bile duct-like structures 7 wk after transplantation. The inoculated hepatocytes possessed HAM4 antigen, which was not recognized on fetal hepatocytes at day 14 of gestation.(ABSTRACT TRUNCATED AT 250 WORDS)
使用抗大鼠肝细胞的单克隆抗体，通过免疫组织化学方法分析了同基因异位移植大鼠肝细胞上膜抗原的表达。分离的成年和胎儿肝细胞在移植后的不同时间段内，能够在脾脏、唾液腺、胸腺或肾被膜下区域存活。在移植到同基因脾脏中的肝细胞上观察到HAM2、4和8抗原相当清晰的表达，这表明这些细胞在功能上可能等同于原位肝细胞。HAM4抗原特异性定位于肝细胞新形成的胆小管面上。移植肝细胞上的HAM2（MHC I类）抗原在面向淋巴组织的一侧表达似乎比其他面更强，这表明肝细胞与淋巴组织之间可能发生了一些免疫反应。在大鼠肝脏中定位于相邻肝细胞间缝隙连接上的HAM8抗原，在移植的肝细胞之间也能被识别。在肝细胞被移植的唾液腺中，不仅在相邻肝细胞之间，而且在肝细胞与唾液腺泡细胞之间都观察到了胆小管样结构，这表明两种不同上皮细胞类型之间有良好的相互作用。移植到胸腺中的肝细胞看起来是存活的，但大多数表现出脂肪变性。一些健康的肝细胞在小叶间结缔组织和胸腺皮质组织中存活。当胎儿肝细胞异位移植时，移植后7周它们形成了一个由肝细胞和胆管样结构组成的团块。接种的肝细胞具有HAM4抗原，而在妊娠第14天的胎儿肝细胞上未识别到该抗原。（摘要截短于250字）
