Kato K, Hodgson W J, Abraham N G, Onodera K, Imai M, Kasai S, Mito M
Department of Medicine and Gastrointestinal Surgery, New York Medical College, Valhalla 10595, USA.
Cell Transplant. 1996 Jan-Feb;5(1):117-22. doi: 10.1177/096368979600500116.
With the development of transplantation of hepatocytes into the spleen, interest has focused on the metabolic changes associated with hepatocyte proliferation. As these changes are important for drug metabolism in hepatocytes, we examined the expression and inducibility of the cytochrome P450 IIIA family within transplanted hepatocytes. Fetal hepatocytes were harvested at 20 days of gestation from spontaneously hypertensive rats (SHRs) and transplanted into recipient adult SHR spleens. Microscopic examination of the recipient spleens 4 and 10 wk after transplantation revealed masses of hepatocytes with cord-like structures in the red pulp. Proliferating hepatocytes were detected with a bromodeoxyuridine (BrdU) immunohistochemical stain. Immunochemical studies detected cytochromes (cytos) P450 p and P450 HLp in fetal hepatocytes before transplantation without prior induction. And although these cytos were not detected by 10 wk after transplantation, they were induced with dexamethasone. These results demonstrated that fetal hepatocytes can be transplanted successfully into recipient spleens and suggested that fetal hepatocytes grow in the spleen, similar to the adult hepatocyte response.
随着脾内肝细胞移植技术的发展，人们的兴趣集中在了与肝细胞增殖相关的代谢变化上。由于这些变化对肝细胞中的药物代谢很重要，我们研究了移植肝细胞中细胞色素P450 IIIA家族的表达及诱导性。从妊娠20天的自发性高血压大鼠（SHR）中获取胎肝细胞，并将其移植到成年受体SHR的脾脏中。移植后4周和10周对受体脾脏进行显微镜检查，结果显示红髓中有大量具有索状结构的肝细胞。用溴脱氧尿苷（BrdU）免疫组化染色检测到增殖的肝细胞。免疫化学研究在移植前未进行预先诱导的胎肝细胞中检测到细胞色素（cytos）P450 p和P450 HLp。虽然移植后10周未检测到这些细胞色素，但用地塞米松可诱导其产生。这些结果表明，胎肝细胞能够成功移植到受体脾脏中，并提示胎肝细胞在脾脏中生长，类似于成年肝细胞的反应。
