Alameddine H S, Louboutin J P, Dehaupas M, Sébille A, Fardeau M
INSERM U 153-CNRS URA 614, Paris, France.
Cell Transplant. 1994 Jan-Feb;3(1):3-14. doi: 10.1177/096368979400300103.
Grafting autologous cultured satellite cells in irreversibly injured rat extensor digitorum longus EDL muscle leads to myofiber regeneration at the grafting site. In this study, we investigated whether cell grafts induced functional improvement and correlated mechanophysiological findings with histological observations. In cell grafted muscles, the number of myofibers did not differ significantly between 2 wk and 3 mo, whereas no regenerating myofibers were observed in ungrafted controls. During this period, the total number of myofibers in the cell grafted muscles represented 48.2-51.9% of that in normal muscles. The mean diameter of regenerated myofibers increased with time, reaching a maximum (32 microns) at the second mo and remained smaller than that of normal myofibers (47 microns). Muscle function was measured by mechanophysiological recordings of muscle response to supramaximal electrical stimulation of the nerve in situ. Cell grafted muscles exhibited a progressive improvement of all contractile parameters. After 3 mo, a 4-fold increase in absolute values of twitch and tetanic tension outputs was measured in cell grafted muscles when compared to ungrafted controls. However, these parameters remained much lower than in normal muscles (23.4% and 22.3% of control, respectively). This study showed that myogenic cell grafts replace degenerated myofibers and form functional myofibers. Functional improvement observed, between 2 wk and 3 mo after cell grafting, correlated with the development, differentiation, and maturation of the regenerated myofibers rather than with an increase in the number of regenerated myofibers.
将自体培养的卫星细胞移植到不可逆损伤的大鼠趾长伸肌（EDL）中，可导致移植部位的肌纤维再生。在本研究中，我们调查了细胞移植是否能诱导功能改善，并将相关的机械生理结果与组织学观察结果进行关联。在细胞移植的肌肉中，2周和3个月时肌纤维数量无显著差异，而未移植的对照组中未观察到再生肌纤维。在此期间，细胞移植肌肉中的肌纤维总数占正常肌肉的48.2 - 51.9%。再生肌纤维的平均直径随时间增加，在第二个月达到最大值（32微米），且仍小于正常肌纤维（47微米）。通过对原位神经进行超强电刺激时肌肉反应的机械生理记录来测量肌肉功能。细胞移植的肌肉在所有收缩参数上均表现出逐渐改善。3个月后，与未移植的对照组相比，细胞移植肌肉的单收缩和强直收缩张力输出绝对值增加了4倍。然而，这些参数仍远低于正常肌肉（分别为对照组的23.4%和22.3%）。本研究表明，成肌细胞移植可替代退化的肌纤维并形成功能性肌纤维。细胞移植后2周和3个月间观察到的功能改善与再生肌纤维的发育、分化和成熟相关，而非与再生肌纤维数量的增加相关。
