海洋单细胞藻类绿色扁藻质膜中的ATP驱动型钠泵。

The ATP-driven Na(+)-pump in the plasma membrane of the marine unicellular alga, Platymonas viridis.

作者信息

Balnokin Y V, Popova L G

机构信息

Laboratory of Salt Exchange and Salt Tolerance, Russian Academy of Science, Moscow.

出版信息

FEBS Lett. 1994 Apr 18;343(1):61-4. doi: 10.1016/0014-5793(94)80607-1.

DOI:10.1016/0014-5793(94)80607-1

PMID:8163019

Abstract

The ATP-supported 22NA+ uptake by plasma membrane vesicles from the marine microalga, Platymonas viridis, was studied. At pH 7 in the medium, Na+ uptake did not occur in the presence of ATP although delta pH across the plasma membrane was generated. The ATP-dependent Na+ uptake was induced by adding the protonophore, ClCCP. At pH 8, Na+ uptake took place when ATP was added even without ClCCP. The delta pH generated across the plasma membrane was negligible under these conditions. The Na+ uptake at pH 8 was not affected by ClCCP and amiloride, an inhibitor of the Na+/H+ antiporter. It is concluded that the ATP-supported Na+ uptake by Pl. viridis vesicles is catalyzed by Na(+)-ATPase.

摘要

对来自海洋微藻绿色扁藻的质膜囊泡进行了由ATP支持的22Na+摄取研究。在培养基pH值为7时，尽管质膜两侧产生了ΔpH，但在ATP存在的情况下，Na+摄取并未发生。添加质子载体羰基氰化物间氯苯腙（ClCCP）可诱导ATP依赖的Na+摄取。在pH值为8时，即使不添加ClCCP，添加ATP时也会发生Na+摄取。在这些条件下，质膜两侧产生的ΔpH可忽略不计。pH值为8时的Na+摄取不受ClCCP和Na+/H+反向转运体抑制剂氨氯吡脒的影响。得出的结论是，绿色扁藻囊泡由ATP支持的Na+摄取是由Na(+)-ATPase催化的。