溶藻弧菌亚细胞囊泡中由ATP驱动的初级钠离子泵

The ATP-driven primary Na+ pump in subcellular vesicles of Vibrio alginolyticus.

作者信息

Dibrov P A, Skulachev V P, Sokolov M V, Verkhovskaya M L

机构信息

Department of Bioenergetics, A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.

出版信息

FEBS Lett. 1988 Jun 20;233(2):355-8. doi: 10.1016/0014-5793(88)80459-9.

DOI:10.1016/0014-5793(88)80459-9

PMID:2968282

Abstract

Subcellular vesicles of Vibrio alginolyticus hydrolyze ATP and accumulate Na+ in an ATP-dependent fashion. The Na+ uptake is (i) strongly stimulated by delta psi-discharging agents, i.e., the protonophorous uncoupler CCCP or valinomycin + K+ and (ii) arrested by DCCD at a concentration strongly inhibiting ATP hydrolysis. Lower concentrations of DCCD stimulate the Na+ accumulation supported by ATP hydrolysis as well as by NADH oxidation. It is concluded that there is an electrogenic DCCD-sensitive Na+-ATPase in the cytoplasmic membrane of V. alginolyticus.

摘要

溶藻弧菌的亚细胞囊泡以ATP依赖的方式水解ATP并积累Na⁺。Na⁺的摄取：(i) 受Δψ消散剂强烈刺激，即质子载体解偶联剂CCCP或缬氨霉素 + K⁺；(ii) 在强烈抑制ATP水解的浓度下被DCCD阻断。较低浓度的DCCD刺激由ATP水解以及NADH氧化支持的Na⁺积累。得出结论，溶藻弧菌细胞质膜中存在一种对DCCD敏感的电生Na⁺-ATP酶。