Agrawal S, Sharma R K, Kishore R, Agarwal S S
Department of Medical Genetics, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow.
Indian J Med Res. 1994 Jan;99:42-6.
Thirty one pregnant women whose sera were initially positive for anti HLA antibodies were retested for lymphocytotoxicity 7-9 months after delivery. In the second testing 10 women were found to have lost the cytotoxic antibodies. These sera were tested for presence of anti-idiotypic antibodies by complement dependent cytotoxicity (CDC). In all ten sera tested, CDC blocking antibody was detected by inhibition technique at different dilutions. These antibodies directed specifically against the HLA antigen of the spouse. This CDC blocking factor could be the anti-idiotypic antibody (Ab2) directed against the cytotoxic anti-HLA antibody (Ab1). In one woman who conceived a second time, repeat sample obtained at the 7th month of the second pregnancy showed reappearance of the specific anti-HLA antibody. This may suggest that the Ab1-Ab2 network is under dynamic regulation.
31名血清抗HLA抗体最初呈阳性的孕妇在分娩后7 - 9个月重新进行淋巴细胞毒性检测。第二次检测时发现10名女性的细胞毒性抗体消失。通过补体依赖细胞毒性（CDC）检测这些血清中是否存在抗独特型抗体。在所有检测的10份血清中，通过抑制技术在不同稀释度下检测到CDC阻断抗体。这些抗体特异性针对配偶的HLA抗原。这种CDC阻断因子可能是针对细胞毒性抗HLA抗体（Ab1）的抗独特型抗体（Ab2）。在一名再次怀孕的女性中，第二次怀孕第7个月采集的重复样本显示特异性抗HLA抗体再次出现。这可能表明Ab1 - Ab2网络处于动态调节之中。
