Heikinheimo K
Department of Oral Pathology, University of Turku, Finland.
J Dent Res. 1994 Mar;73(3):590-7. doi: 10.1177/00220345940730030401.
Members of the decapentaplegic-Vg-related (DVR) gene family are diffusible signaling molecules regulating inductive tissue interactions during vertebrate development. Expression of DVR/bone morphogenetic protein (BMP) 2, 4, and 6 was studied in human fetal teeth. Sequential morphogenetic stage-specific studies of DVR/BMP 2 and 4 mRNA expression by in situ hybridization revealed transcripts for DVR/BMP 4 during compaction of the dental mesenchyme. In contrast, DVR/BMP 2 mRNA appeared later during tooth development and was located in differentiated cells (odontoblasts). These results were confirmed by reverse-transcription polymerase chain reaction (RT-PCR), which detected DVR/BMP 2 and 4 mRNA in human tooth-germ samples. DVR/BMP 6 protein was distributed in the early dental epithelium and, later, in pre-odontoblasts and odontoblasts, where it remained during dentin formation. These results suggest that DVR/BMP 4 is involved in the early tooth morphogenesis. DVR/BMP 6 may, in particular, be implicated in epithelial-mesenchymal interactions controlling cytodifferentiation. DVR/BMP 2 and 6 may also be involved in odontoblast secretory function. The results suggest that members of the DVR gene family may play regulatory roles during human tooth development.
十五体瘫-Vg相关（DVR）基因家族的成员是可扩散的信号分子，在脊椎动物发育过程中调节诱导性组织相互作用。研究了人胎儿牙齿中DVR/骨形态发生蛋白（BMP）2、4和6的表达。通过原位杂交对DVR/BMP 2和4 mRNA表达进行的连续形态发生阶段特异性研究显示，在牙间充质压实过程中存在DVR/BMP 4转录本。相比之下，DVR/BMP 2 mRNA在牙齿发育后期出现，位于分化细胞（成牙本质细胞）中。这些结果通过逆转录聚合酶链反应（RT-PCR）得到证实，该反应在人牙胚样本中检测到了DVR/BMP 2和4 mRNA。DVR/BMP 6蛋白分布在早期牙上皮中，随后分布在成牙本质细胞前体和成牙本质细胞中，并在牙本质形成过程中一直存在。这些结果表明，DVR/BMP 4参与早期牙齿形态发生。DVR/BMP 6尤其可能参与控制细胞分化的上皮-间充质相互作用。DVR/BMP 2和6也可能参与成牙本质细胞的分泌功能。结果表明，DVR基因家族成员可能在人类牙齿发育过程中发挥调节作用。
