Ikegawa S, Goto J
Pharmaceutical Institute, Tohoku, University.
Nihon Rinsho. 1994 Mar;52(3):660-5.
Gas chromatography/mass spectrometry (GC/MS) has been used as a sensitive and specific tool for the determination of steroid hormones. The instruments made great progress in the advent of chemically inert fused silica capillary columns and various sample injection systems. Characteristics of electron impact and chemical ionization methods, and of the derivatization reagent are described. The sensitive method by GC in combination with negative ion chemical ionization MS is also outlined. Solid phase extraction by an octadecylsilyl bonded silica cartridge and an immobilized antibody column as well as chromatographic separation by lipophilic ion-exchange gel for the clean-up of steroid hormones in biological fluids are discussed in this article.
气相色谱/质谱联用（GC/MS）已被用作测定甾体激素的灵敏且特异的工具。在化学惰性熔融石英毛细管柱和各种进样系统出现后，这些仪器取得了巨大进展。文中描述了电子轰击和化学电离方法以及衍生化试剂的特点。还概述了气相色谱结合负离子化学电离质谱的灵敏方法。本文讨论了使用十八烷基硅烷键合硅胶柱和固定化抗体柱进行固相萃取，以及使用亲脂性离子交换凝胶进行色谱分离以净化生物流体中甾体激素的方法。
