Burns F J, Vanderlaan M, Sivak A, Albert R E
Cancer Res. 1976 Apr;36(4):1422-7.
The persistence and proliferation rate of mouse skin papillomas were studied in HA/ICR mice initiated with 7,12-dimethylbenz(a)anthracene and promoted three times weekly with phorbol myristate acetate. When the promoter treatments were stopped, rapid (half-time, 24 days) and slow (half-time, greater than 140 days) components of papilloma regression were observed. When the promoter dose was increased, the major effect was an increase among the rapidly regressing papillomas. Increases in the epidermal pulse-labeling index and the number of dermal inflammatory cells produced by phorbol myristate acetate in normal skin were reversible when the phorbol myristate acetate was stopped, but high pulse-labeling index values in papillomas were not reversible. Antithymocyte serum had no effect on regression, although ethylphenylpropriolate, a nonpromoting irritant, slowed the regression sufficiently to increase the half-time from 24 to 57 days. The action of the promoter in overcoming the regression tendency of the papillomas may explain certain features of the role of nonspecific irritation and the importance of promotion frequency in determining tumor yield.
在以7,12 - 二甲基苯并(a)蒽引发并每周用佛波酯肉豆蔻酸酯促进三次的HA/ICR小鼠中，研究了小鼠皮肤乳头瘤的持续存在和增殖率。当停止促进剂处理时，观察到乳头瘤消退的快速（半衰期为24天）和缓慢（半衰期大于140天）成分。当增加促进剂剂量时，主要影响是快速消退的乳头瘤数量增加。当停止使用佛波酯肉豆蔻酸酯时，正常皮肤中由佛波酯肉豆蔻酸酯产生的表皮脉冲标记指数和真皮炎症细胞数量的增加是可逆的，但乳头瘤中的高脉冲标记指数值是不可逆的。抗胸腺细胞血清对消退没有影响，尽管非促进性刺激物乙基苯基丙炔酸酯足以减缓消退，使半衰期从24天增加到57天。促进剂克服乳头瘤消退趋势的作用可能解释了非特异性刺激作用的某些特征以及促进频率在确定肿瘤产量中的重要性。
