Takeshita S, Kawase H, Yamamoto M, Fujisawa T, Sekine I, Yoshioka S
Department of Pediatrics, National Defense Medical College, Tokorozawa, Japan.
Pediatr Res. 1994 Feb;35(2):179-83. doi: 10.1203/00006450-199402000-00010.
To investigate the possible role of heat shock protein (HSP) in the pathogenesis of Kawasaki disease (KD), we determined the expression of 63-kD HSP (P1 protein) gene in the leukocytes from the peripheral blood of 20 patients with KD, 20 patients with various other febrile diseases, and 10 healthy children using the quantitative reverse transcription-polymerase chain reaction technique. Although a low but constitutive expression of HSP was observed in the control patients and healthy children, the expression of HSP63 mRNA was significantly higher during the acute phase of KD. Because mycobacterial HSP65, a cognate of human HSP63, is a common immunogen in bacterial species, and because the major immunopathologic feature of KD is reportedly an abnormally activated immune system, it suggests that an increased level of HSP63 gene transcription may be associated with the pathogenesis of KD.
为了研究热休克蛋白（HSP）在川崎病（KD）发病机制中的可能作用，我们采用定量逆转录-聚合酶链反应技术，测定了20例KD患者、20例其他各种发热性疾病患者以及10名健康儿童外周血白细胞中63-kD HSP（P1蛋白）基因的表达。尽管在对照患者和健康儿童中观察到HSP有低水平的组成性表达，但HSP63 mRNA在KD急性期的表达显著更高。由于人HSP63的同源物——分枝杆菌HSP65是细菌中的一种常见免疫原，且据报道KD的主要免疫病理特征是免疫系统异常激活，这表明HSP63基因转录水平升高可能与KD的发病机制有关。
