Department of Pediatrics, Juntendo University School of Medicine, Tokyo, Japan.
Immunology. 2009 Dec;128(4):511-20. doi: 10.1111/j.1365-2567.2009.03135.x.
We previously suggested that gut bacteria may be involved in the onset of Kawasaki disease (KD). In this study, we evaluated the production of heat shock proteins (hsps) and superantigens (sAgs) by microorganisms isolated from the jejunal mucosa of 19 children with KD in the acute phase and from 15 age-matched control children. We identified 13 strains of Gram-negative microbes from patients with KD; these microbes produced large amounts of hsp60 and induced pro-inflammatory cytokine production by peripheral blood mononuclear cells. The Gram-negative microbes also elicited endogenous hsp60 production, leading to the secretion of anti-inflammatory intereukin-10 (IL-10). We also identified 18 strains of Gram-positive cocci that had superantigenic properties and which induced the expansion of Vbeta2 T cells in vitro. All bacteria identified in this study were antibiotic resistant. These data suggest that sAg and hsps produced by gut bacteria might be involved in KD.
我们之前曾提出肠道细菌可能与川崎病（KD）的发病有关。在这项研究中，我们评估了从 19 例川崎病急性期患儿和 15 例年龄匹配的对照组患儿空肠黏膜分离的微生物产生热休克蛋白（hsps）和超抗原（sAgs）的情况。我们从川崎病患儿中鉴定出 13 株革兰氏阴性微生物，这些微生物大量产生 hsp60，并诱导外周血单个核细胞产生促炎细胞因子。革兰氏阴性微生物还引发内源性 hsp60 的产生，导致抗炎细胞因子白细胞介素-10（IL-10）的分泌。我们还鉴定出 18 株具有超抗原特性的革兰氏阳性球菌，这些球菌在体外诱导 Vbeta2 T 细胞的扩增。本研究中鉴定出的所有细菌均具有抗药性。这些数据表明肠道细菌产生的 sAg 和 hsps 可能与 KD 有关。
