大肠杆菌中II组内含子插入与移动DNA序列相关的高度保守阅读框的证据。
Evidence for a group II intron in Escherichia coli inserted into a highly conserved reading frame associated with mobile DNA sequences.
作者信息
Knoop V, Brennicke A
机构信息
Institut für Genbiologische Forschung, Berlin, Germany.
出版信息
Nucleic Acids Res. 1994 Apr 11;22(7):1167-71. doi: 10.1093/nar/22.7.1167.
Abstract
The distribution of group II introns in the living world is an important aspect of the hypothesis which postulates their evolutionary relation to the nuclear spliceosome. As an alternative to the restricted experimental approaches towards their identification we devised a strategy to recognize group II introns in sequence data. By this approach we identified a locus on a plasmid in the bacterium Escherichia coli. Modelling of the derived RNA secondary structure reveals the presence of perfectly conserved domains V and VI as typical features of group II introns. An intron internal reading frame upstream of domain V is homologous to group II intron encoded maturases. A reading frame downstream of the predicted 3'-splice site is highly similar to a small polypeptide encoded in the central part of the Agrobacterium tumefaciens T-DNA. With the TBLASTN algorithm a set of plasmid-borne insertion sequences in Agrobacteria and Rhizobia and surprisingly also in a Yersinia pseudotuberculosis strain was identified which contain this highly conserved reading frame.
摘要
II类内含子在生物界的分布是假定其与核剪接体存在进化关系这一假说的重要方面。作为识别它们的有限实验方法的替代方法，我们设计了一种在序列数据中识别II类内含子的策略。通过这种方法，我们在大肠杆菌的一个质粒上鉴定出一个位点。对推导的RNA二级结构进行建模，揭示出存在完全保守的结构域V和VI，这是II类内含子的典型特征。结构域V上游的一个内含子内部阅读框与II类内含子编码的成熟酶同源。预测的3'剪接位点下游的一个阅读框与根癌农杆菌T-DNA中部编码的一个小多肽高度相似。利用TBLASTN算法，在农杆菌和根瘤菌中，令人惊讶的是在一株假结核耶尔森氏菌中，鉴定出一组携带质粒的插入序列，它们含有这个高度保守的阅读框。
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