Fox T D, Leaver C J
Cell. 1981 Nov;26(3 Pt 1):315-23. doi: 10.1016/0092-8674(81)90200-2.
Cross hybridization between maize mitochondrial DNA fragments and a specific yeast mitochondrial DNA probe from the oxi 1 gene has been used to identify and isolate the maize mitochondrial gene coding cytochrome oxidase subunit II, mox 1. The DNA sequence reveals two coding regions separated from each other by a single centrally located intervening sequence. Hybridization of mox 1 DNA probes to mitochondrial RNA from plants shows that te gene is transcribed and indicates that several transcripts are spliced. TGA codons, which code Trp in the mitochondria of all species examined to date, do not occur in this gene. However, alignment of the mox 1 gene sequence with the amino acid sequences of subunit II from other organisms strongly suggests that codon CGG (normally Arg) codes for Trp in maize mitochondria, in addition to the standard Trp codon TGG.
利用玉米线粒体DNA片段与来自酵母oxi 1基因的特定线粒体DNA探针之间的交叉杂交，已鉴定并分离出编码细胞色素氧化酶亚基II的玉米线粒体基因mox 1。DNA序列显示有两个编码区，它们被一个位于中心位置的单一间隔序列隔开。mox 1 DNA探针与植物线粒体RNA的杂交表明该基因被转录，并表明有几种转录本被剪接。TGA密码子在迄今为止所检测的所有物种的线粒体中都编码色氨酸，但在该基因中并不出现。然而，mox 1基因序列与其他生物的亚基II氨基酸序列的比对强烈表明，除了标准的色氨酸密码子TGG外，密码子CGG（通常编码精氨酸）在玉米线粒体中编码色氨酸。
