Smith G, McGimpsey W G, Lynch M C, Kochevar I E, Redmond R W
Department of Chemistry, Worcester Polytechnic Institute, MA 01609.
Photochem Photobiol. 1994 Feb;59(2):135-9. doi: 10.1111/j.1751-1097.1994.tb05012.x.
A novel oxygen-independent photosensitization mechanism from the upper triplet state (Tn) of rose bengal has been demonstrated by selectively populating Tn by sequential two-color laser excitation. Products formed from Tn inhibit red blood cell acetylcholinesterase and decrease viability of P388D1 mouse macrophage monocyte cells as measured by trypan blue exclusion assay. Laser flash photolysis studies indicate that Tn reacts efficiently, as evidenced by permanent photobleaching of T1 absorption, with chemical yields approaching unit efficiency. This mechanism may have application for oxygen deficient photosensitization under high intensity, pulsed laser irradiation.
通过双色激光顺序激发选择性地填充孟加拉玫瑰红的三重态（Tn），已证明了一种来自其三重态的新型不依赖氧气的光敏化机制。由Tn形成的产物可抑制红细胞乙酰胆碱酯酶，并通过台盼蓝排斥试验测定，降低P388D1小鼠巨噬细胞单核细胞的活力。激光闪光光解研究表明，Tn反应高效，T1吸收的永久性光漂白证明了这一点，化学产率接近单位效率。这种机制可能适用于高强度脉冲激光照射下的缺氧光敏化。
