Ferguson J E, Seaner R, Bruns D E, Redick J A, Mills S E, Jüppner H, Segre G V, Bruns M E
Department of Obstetrics and Gynecology, University of Virginia School of Medicine, Charlottesville.
Am J Obstet Gynecol. 1994 Apr;170(4):1018-24; discussion 1024-6. doi: 10.1016/s0002-9378(94)70095-8.
Our purpose was to study the expression and localization of parathyroid hormone-related protein and expression of the parathyroid hormone/parathyroid hormone-related protein receptor in human umbilical cord.
The expression and localization of parathyroid hormone-related protein and expression of the parathyroid hormone/parathyroid hormone-related protein receptor were studied in isolated tissues from the human umbilical cord by Northern analysis, reverse-transcriptase polymerase chain reaction, Southern gel analysis, and immunolocalization procedures at the light and electron microscopic levels.
Parathyroid hormone-related protein was abundantly expressed in the umbilical cord. Immunohistochemical and immunocytochemical techniques confirmed hormone localization in the amnion epithelial layer and in vascular smooth muscle and endothelial cells in vessels from the umbilical cord and placental chorionic plate. Multiplex reverse-transcriptase polymerase chain reaction identified expression of receptor messenger ribonucleic acid in vessels of the umbilical cord; this finding was verified by means of Southern gel analysis of the products of the reverse-transcriptase polymerase chain reaction.
A parathyroid hormone-related protein paracrine system appears to exist in human umbilical cord. We suggest that it may be involved in the control of fetal placental circulation.
我们的目的是研究甲状旁腺激素相关蛋白在人脐带中的表达及定位，以及甲状旁腺激素/甲状旁腺激素相关蛋白受体的表达。
通过Northern分析、逆转录聚合酶链反应、Southern凝胶分析以及光镜和电镜水平的免疫定位程序，研究甲状旁腺激素相关蛋白在人脐带分离组织中的表达及定位，以及甲状旁腺激素/甲状旁腺激素相关蛋白受体的表达。
甲状旁腺激素相关蛋白在脐带中大量表达。免疫组织化学和免疫细胞化学技术证实该激素定位于羊膜上皮层、脐带和胎盘绒毛板血管的血管平滑肌和内皮细胞中。多重逆转录聚合酶链反应确定了脐带血管中受体信使核糖核酸的表达；这一发现通过对逆转录聚合酶链反应产物的Southern凝胶分析得到证实。
人脐带中似乎存在甲状旁腺激素相关蛋白旁分泌系统。我们认为它可能参与胎儿胎盘循环的调控。
