转化生长因子-β1信使核糖核酸在人子宫内膜细胞中的表达及转化生长因子-β1对脱氧核糖核酸合成的调节

Expression of transforming growth factor-beta 1 messenger ribonucleic acid and the modulation of deoxyribonucleic acid synthesis by transforming growth factor-beta 1 in human endometrial cells.

作者信息

Marshburn P B, Arici A M, Casey M L

机构信息

Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center at Dallas 75235-9032.

出版信息

Am J Obstet Gynecol. 1994 Apr;170(4):1152-8. doi: 10.1016/s0002-9378(94)70112-1.

DOI:10.1016/s0002-9378(94)70112-1

PMID:8166201

Abstract

OBJECTIVE

The purpose of this study was (1) to evaluate the potential sites of transforming growth factor-beta 1 synthesis in human endometrium by analyzing separated endometrial glands and stromal cells for transforming growth factor-beta 1 messenger ribonucleic acid by Northern analysis of total ribonucleic acid and (2) to investigate the effects of transforming growth factor-beta 1 on deoxyribonucleic acid synthesis in endometrial epithelial and stromal cells in culture.

STUDY DESIGN

Endometrial glands and stroma from proliferative and secretory endometrium were isolated after collagenase treatment of endometrial tissue minces and were analyzed for transforming growth factor-beta 1 messenger ribonucleic acid by Northern analysis. We studied the effects of estradiol-17 beta and transforming growth factor-beta 1 on deoxyribonucleic acid synthesis in endometrial epithelium and transforming growth factor-beta 1 on stromal cells in culture by evaluating tritiated thymidine incorporation into trichloroacetic acid-precipitable material.

RESULTS

Transforming growth factor-beta 1 messenger ribonucleic acid was detected for Northern analysis in separated endometrial stromal cells in levels that were greatest during the secretory phase and in greater levels than in epithelial cells from that same tissue. Transforming growth factor-beta 1 messenger ribonucleic acid in glandular epithelium in culture was not increased to detectable levels by treatment with transforming growth factor-beta 1. Deoxyribonucleic acid synthesis in endometrial glandular epithelium was inhibited by transforming growth factor-beta 1, but transforming growth factor-beta 1 stimulated deoxyribonucleic acid synthesis in endometrial stromal cells in culture. After treatment for 5 days with estradiol-17 beta (10(-8) mol/L), deoxyribonucleic acid synthesis in endometrial glands in culture was decreased by 40%. Transforming growth factor-beta 1 (1 ng/ml) did not alter this effect of estradiol-17 beta on deoxyribonucleic acid synthesis.

CONCLUSIONS

Transforming growth factor-beta 1 acts to decrease deoxyribonucleic acid synthesis in epithelial cells and to increase it in stromal cells isolated from human endometrium and maintained in monolayer culture. Transforming growth factor-beta 1, potentially of stromal cell origin, could participate in the regulation of endometrial cell proliferation and differentiation in vivo.

摘要

目的

本研究的目的是：（1）通过对分离的子宫内膜腺体和基质细胞的总核糖核酸进行Northern分析，检测转化生长因子-β1信使核糖核酸，以评估人子宫内膜中转化生长因子-β1的潜在合成部位；（2）研究转化生长因子-β1对培养的子宫内膜上皮和基质细胞中脱氧核糖核酸合成的影响。

研究设计

用胶原酶处理子宫内膜碎块后，分离增殖期和分泌期子宫内膜的腺体和基质，并用Northern分析检测转化生长因子-β1信使核糖核酸。通过评估氚标记胸腺嘧啶掺入三氯乙酸沉淀物质的情况，我们研究了雌二醇-17β和转化生长因子-β1对培养的子宫内膜上皮细胞中脱氧核糖核酸合成的影响，以及转化生长因子-β1对培养的基质细胞的影响。

结果

在分离的子宫内膜基质细胞中检测到转化生长因子-β1信使核糖核酸，其水平在分泌期最高，且高于同一组织的上皮细胞中的水平。用转化生长因子-β1处理培养的腺上皮细胞，其转化生长因子-β1信使核糖核酸未增加到可检测水平。转化生长因子-β1抑制子宫内膜腺上皮细胞中的脱氧核糖核酸合成，但刺激培养的子宫内膜基质细胞中的脱氧核糖核酸合成。用雌二醇-17β（10⁻⁸mol/L）处理5天后，培养的子宫内膜腺体中的脱氧核糖核酸合成减少了40%。转化生长因子-β1（1ng/ml）未改变雌二醇-17β对脱氧核糖核酸合成的这种作用。