Fujimoto S, Takahashi M, Kiuchi S, Kobayashi K, Mutou T, Masaoka H, Shimanskaya R B, Ohkubo H
Social Insurance Funabashi Central Hospital, Funabashi, Japan.
Anticancer Res. 1994 Jan-Feb;14(1A):67-71.
Hyperthermia-induced antitumor activity was assessed histopathologically and findings related to transplantation of human gastric cancer into nude mice were examined. Fragments of human gastric cancer were incubated at 37 degrees C to 47 degrees C for various durations of time, then were evaluated either histologically or with regard to delay in tumor growth and the rates of transplantation into nude mice. Fragments exposed to 39 degrees C for 30 min to 120 min and 41 degrees C for 30 min did not differ from findings in the controls concerning tumor growth and transplantability. In the case of 41 degrees C for 60 min or more, the rates of transplantation decreased significantly and there was a delay in tumor growth. At 43 degrees C for 120 min, 45 degrees C for 30 and 60 min, and 47 degrees C for 15 to 60 min, transplantability was nil. In the groups with a delay in tumor growth, there were irreversible changes in nucleic and cytoplasmic components, and in 6 groups with no evidence of transplantability, complete destruction of the glandular structure, pyknosis and karyorrhexis as well as disappearance of the cytoplasm occurred in almost all cases. Thus, the thermal dose of 43 degrees C for 120 min seems to be the minimally effective one for hyperthermia-induced tumoricidal activity.
通过组织病理学评估热疗诱导的抗肿瘤活性，并检查与人类胃癌移植到裸鼠体内相关的结果。将人类胃癌组织块在37摄氏度至47摄氏度下孵育不同时长，然后从组织学角度或根据肿瘤生长延迟情况以及移植到裸鼠体内的成功率进行评估。暴露于39摄氏度30分钟至120分钟以及41摄氏度30分钟的组织块，在肿瘤生长和可移植性方面与对照组的结果没有差异。在41摄氏度处理60分钟及以上的情况下，移植成功率显著下降，肿瘤生长出现延迟。在43摄氏度处理120分钟、45摄氏度处理30分钟和60分钟以及47摄氏度处理15分钟至60分钟的情况下，可移植性为零。在肿瘤生长出现延迟的组中，核酸和细胞质成分发生了不可逆变化，在6组无可移植性证据的组中，几乎所有病例都出现了腺结构的完全破坏、核固缩和核碎裂以及细胞质消失。因此，43摄氏度处理120分钟的热剂量似乎是热疗诱导杀瘤活性的最低有效剂量。
