枯草芽孢杆菌各种产孢和不产孢突变体中互补脱氧核糖核酸链的转录。对热敏感产孢突变体（ts-4）体内合成的核糖核酸进行的杂交竞争研究。

Transcription from complementary deoxyribonucleic acid strands in various sporogenic and asporogenic mutants of Bacillus subtilis. Hybridization-competition studies on ribonucleic acid synthesized in vivo by a thermosensitive sporulation mutant (ts-4).

作者信息

Bonamy C, Manca de Nadra M C, Szulmajster J

出版信息

Eur J Biochem. 1976 Mar 16;63(1):53-63. doi: 10.1111/j.1432-1033.1976.tb10206.x.

DOI:10.1111/j.1432-1033.1976.tb10206.x

Abstract

The present paper describes an investigation, at the transcription level, in a thermosensitive sporulation mutant of Bacillus subtilis (ts-4) grown at the permissive (30 degrees C) or restrictive (42 degrees C) temperature where sporulation capacity is respectively expressed or arrested. These studies were carried out by analysing the ribonucleic acid from vegetative and stationary phase cells (t3 cells) grown under both conditions, by hybridization-competition experiments.

摘要

本文描述了在转录水平上对枯草芽孢杆菌的一个热敏性芽孢形成突变体（ts - 4）进行的研究。该突变体在允许温度（30摄氏度）或限制温度（42摄氏度）下生长，在这两种温度下芽孢形成能力分别得到表达或受到抑制。这些研究通过杂交竞争实验，分析在这两种条件下生长的营养细胞和稳定期细胞（t3细胞）的核糖核酸来进行。