Sabbah A, Hassoun S, Drouet M, Lauret M G
Laboratoire d'Immuno-Allergologie, CHU, Angers.
Allerg Immunol (Paris). 1994 Jan;26(1):2-5.
Objective improvement in the diagnosis of immuno-allergology can only be obtained by application of reliable and reproducible immuno-biological methods, but until now, only measurements of total and specific IgE can be used and this has certain limitations. Presence of specific serum IgE, correlated with skin tests, favours a sensitization and implies nothing about the responsibility of the allergens. This is why we must consider if a definite improvement of diagnostic methods can be obtained by measurement of mediators. From an observation of food allergy to pork meat, we now show that it is possible to use sequential measurements of the mediators plasma histamine and urinary methylhistamine, ECP and serum tryptase to refine the diagnosis and provide proof of the responsibility of the food allergen. We report here a didactic observation which is characterised by reproducibility and specificity of the measurements. It illustrates the progress in diagnostic methods in allergy: we give a statistical diagnosis by measurement of antibodies and a dynamic diagnosis by measurement of mediators.
免疫过敏学诊断的客观改善只能通过应用可靠且可重复的免疫生物学方法来实现，但到目前为止，只能使用总IgE和特异性IgE的检测，而这存在一定局限性。特异性血清IgE的存在与皮肤试验相关，提示致敏，但对于变应原的责任并无说明。这就是为什么我们必须考虑通过检测介质是否能够实现诊断方法的明确改进。通过对猪肉食物过敏的观察，我们现在表明，连续检测血浆组胺和尿甲基组胺、嗜酸性粒细胞阳离子蛋白（ECP）和血清类胰蛋白酶，可用于完善诊断并提供食物变应原责任的证据。我们在此报告一项具有教学意义的观察结果，其特点是检测具有可重复性和特异性。它说明了过敏诊断方法的进展：我们通过检测抗体进行统计学诊断，通过检测介质进行动态诊断。
