Broda P, Birch P, Brooks P, Copa-Patiño J L, Sinnott M L, Tempelaars C, Wang Q, Wyatt A, Sims P
Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, UK.
FEMS Microbiol Rev. 1994 Mar;13(2-3):189-95. doi: 10.1111/j.1574-6976.1994.tb00042.x.
We seek to define more fully how Phanerochaete chrysosporium degrades its natural substrate, lignocellulose. This contribution concerns several relevant topics. Mineralisation of [14C]DHP, as a model for lignin degradation, showed that a set of genetically defined meiotically derived products of strain ME446 differed in their degradative ability and also that, under optimum conditions for mineralisation, extracellular lignin peroxidase activity was absent. Xylanolytic and xylosidase/beta(1-->3) glucanase activities are also described. The complexity of the CBHI gene family is described and differential splicing of a CBHI gene transcript is proposed. In contrast to the multiplicity of CHBI genes there is a single CBHII gene. PCR methods were developed to analyse differential gene expression on different substrates. We have also developed a transformation system involving a reporter construct for the analysis of CBHI promoter function.
我们试图更全面地界定黄孢原毛平革菌如何降解其天然底物木质纤维素。本论文涉及几个相关主题。作为木质素降解模型的[14C]二氢愈创木脂矿化表明，ME446菌株一组经遗传学定义的减数分裂衍生产物在降解能力上存在差异，并且在矿化的最佳条件下，细胞外木质素过氧化物酶活性并不存在。同时还描述了木聚糖酶活性以及木糖苷酶/β(1→3)葡聚糖酶活性。阐述了CBHI基因家族的复杂性，并提出了一个CBHI基因转录本的可变剪接。与多个CHBI基因不同，只有一个CBHII基因。开发了聚合酶链反应方法来分析不同底物上的差异基因表达。我们还开发了一种转化系统，该系统涉及一个用于分析CBHI启动子功能的报告构建体。
