Lamar R T, Schoenike B, Vanden Wymelenberg A, Stewart P, Dietrich D M, Cullen D
Forest Products Laboratory, U.S. Department of Agriculture, Madison, Wisconsin 53705, USA.
Appl Environ Microbiol. 1995 Jun;61(6):2122-6. doi: 10.1128/aem.61.6.2122-2126.1995.
Thorough analysis of fungi in complex substrates has been hampered by inadequate experimental tools for assessing physiological activity and estimating biomass. We report a method for the quantitative assessment of specific fungal mRNAs in soil. The method was applied to complex gene families of Phanerochaete chrysosporium, a white-rot fungus widely used in studies of organopollutant degradation. Among the genes implicated in pollutant degradation, two closely related lignin peroxidase transcripts were detected in soil. The pattern of lignin peroxidase gene expression was unexpected; certain transcripts abundant in defined cultures were not detected in soil cultures. Transcripts encoding cellobiohydrolases and beta-tubulin were also detected. The method will aid in defining the roles of specific genes in complex biological processes such as organopollutant degradation, developing strategies for strain improvement, and identifying specific fungi in environmental samples.
用于评估生理活性和估算生物量的实验工具不足，阻碍了对复杂基质中真菌的全面分析。我们报告了一种定量评估土壤中特定真菌信使核糖核酸（mRNA）的方法。该方法应用于黄孢原毛平革菌（一种广泛用于有机污染物降解研究的白腐真菌）的复杂基因家族。在与污染物降解相关的基因中，在土壤中检测到了两个密切相关的木质素过氧化物酶转录本。木质素过氧化物酶基因的表达模式出乎意料；在特定培养物中丰富的某些转录本在土壤培养物中未被检测到。还检测到了编码纤维二糖水解酶和β-微管蛋白的转录本。该方法将有助于确定特定基因在有机污染物降解等复杂生物过程中的作用，制定菌株改良策略，并鉴定环境样品中的特定真菌。
