Colomb E, Martin P M
Laboratoire de Cancérologie Experimentale, Faculté de Médecine Nord, Marseille, France.
Anal Cell Pathol. 1994 Feb;6(2):105-16.
Until now chemosensitivity of tumour cells has been evaluated solely on the basis of cell growth or survival testing. In order to rapidly evaluate sensitivity to anthracyclines, which are well known to disturb DNA organization, we propose a model based on detection of conformational changes in DNA structure. Chromatin texture changes were assessed using a cell image processor which computes six densitometric and nine texture parameters on each Feulgen-stained nucleus and provides multiparametric analyses of data. The technique was tested on two cell lines of human breast cancer, differing only with regard to their sensitivity to anthracycline. The percentage of nuclei affected by the drug and distribution of the cell cycle phases were determined on the same cells. This method can be used to predict the efficacy of anthracyclines used alone or in conjunction with other drugs. It may also be applicable for other therapeutic agents causing conformational changes in DNA structure.
到目前为止，肿瘤细胞的化学敏感性仅基于细胞生长或存活测试来评估。为了快速评估对已知会干扰DNA组织的蒽环类药物的敏感性，我们提出了一种基于检测DNA结构构象变化的模型。使用细胞图像处理器评估染色质纹理变化，该处理器对每个Feulgen染色的细胞核计算六个光密度参数和九个纹理参数，并提供数据的多参数分析。该技术在两种人乳腺癌细胞系上进行了测试，这两种细胞系仅在对蒽环类药物的敏感性方面有所不同。在相同的细胞上测定受药物影响的细胞核百分比和细胞周期阶段分布。该方法可用于预测单独使用或与其他药物联合使用的蒽环类药物的疗效。它也可能适用于其他导致DNA结构构象变化的治疗剂。
