Tucker J H, Cowpe J G, Ogden G R
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.
Anal Cell Pathol. 1994 Feb;6(2):117-28.
Three methods of DNA content assessment providing both rare-event and population features were applied to forty eight Feulgen-stained oral smears collected from twenty patients. The smears were collected from three sites and diagnosed either clinically as normal, or histologically as premalignant or malignant. Cell morphometry features were also measured from Papanicolaou-stained smears from each site. The three methods of DNA content assessment could distinguish clearly between normal and abnormal smears, but not between premalignant and malignant smears within the abnormal group. However discrimination could be obtained between these subgroups using cytoplasmic area. The results therefore suggest that a combination of DNA content and morphometric measurement of oral smears can give a useful method for detecting and localising premalignant and malignant conditions.
三种用于评估DNA含量的方法，既能提供罕见事件特征又能提供群体特征，被应用于从20名患者收集的48份福尔根染色口腔涂片。涂片取自三个部位，临床诊断为正常，或组织学诊断为癌前病变或恶性病变。还从每个部位的巴氏染色涂片中测量了细胞形态计量学特征。这三种DNA含量评估方法能够清楚地区分正常涂片和异常涂片，但在异常组内无法区分癌前涂片和恶性涂片。然而，使用细胞质面积可以在这些亚组之间进行区分。因此，结果表明，结合口腔涂片的DNA含量和形态计量学测量可以提供一种检测和定位癌前病变和恶性病变的有用方法。
