Schulte E K, Joos U, Kasper M, Eckert H M
Anatomische Anstalt, Universität München, FRG.
Diagn Cytopathol. 1991;7(4):436-41. doi: 10.1002/dc.2840070421.
Cytological scrape material of the oral mucosa from 114 patients with epithelial dysplasia and with oral cancer was stained with the Feulgen-reaction and investigated with an image analyzer. The size and the integrated optical density of cell nuclei, and four chromatin texture features were measured. All tumor slides contained cell nuclei with DNA greater than 5c, 16% of the slides had cell nuclei with DNA greater than 8c. A total of 14.5% of the tumor patients showed significantly increased DNA values in nuclei distant from the tumor. Two smears with severe epithelial dysplasia showed nuclei with DNA greater than 5c both in the tumor material and far from the tumor. Texture analysis allowed discrimination between benign, dysplastic and malignant smears. No correlation was found between DNA content and tumor staging. Image cytometry was a reliable method for detecting tumor cells. Epithelial dysplasia in areas distant from the tumor is probably due to "field canceration" of the epithelium.
对114例上皮发育异常和口腔癌患者的口腔黏膜细胞刮片材料进行福尔根反应染色，并用图像分析仪进行研究。测量了细胞核的大小和积分光密度，以及四个染色质纹理特征。所有肿瘤切片均含有DNA大于5c的细胞核，16%的切片含有DNA大于8c的细胞核。共有14.5%的肿瘤患者在远离肿瘤的细胞核中显示出显著升高的DNA值。两张重度上皮发育异常涂片在肿瘤组织和远离肿瘤的部位均显示出DNA大于5c的细胞核。纹理分析能够区分良性、发育异常和恶性涂片。未发现DNA含量与肿瘤分期之间存在相关性。图像细胞术是检测肿瘤细胞的可靠方法。远离肿瘤区域的上皮发育异常可能是由于上皮的“场癌变”。
