Gańan Y, Macias D, Hurle J M
Departamento de Ciencias Morfologicas, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain.
Dev Dyn. 1994 Jan;199(1):64-72. doi: 10.1002/aja.1001990107.
In previous studies we have observed that the interdigital tissue of the chick embryo leg bud during the stages previous to interdigital cell death exhibits a considerable chondrogenic potentiality both in vivo and in vitro. In the present investigation we have carried out a variety of experimental manipulations of the chick leg bud at stage 29 to discover possible mechanisms accounting for interdigital ectopic chondrogenesis and extradigit formation. Our results show that the interdigital tissue is capable of forming an extradigit when temporarily isolated microsurgically and regrafted in its original location and after deletion of one of the adjacent digital primordia, suggesting that developing phalangeal cartilages exercise an inhibitory effect on chondrification in adjoining tissues. Furthermore, and of greater importance, ablation of the primordium of a digit is followed by normal development of the definitive digit if the wound surfaces are suitably apposed. These results reveal a considerable regulatory potential in the autopodium at advanced stages of development.
在先前的研究中，我们观察到，在趾间细胞死亡之前的阶段，鸡胚腿芽的趾间组织在体内和体外均表现出相当大的软骨形成潜力。在本研究中，我们对处于第29阶段的鸡胚腿芽进行了各种实验操作，以发现可能导致趾间异位软骨形成和额外趾形成的机制。我们的结果表明，当通过显微手术暂时分离趾间组织并将其重新移植到其原始位置，以及在删除相邻指原基之一后，趾间组织能够形成额外趾，这表明正在发育的指骨软骨对相邻组织的软骨化具有抑制作用。此外，更重要的是，如果伤口表面适当贴合，在切除一个指原基后，定型指会正常发育。这些结果揭示了在发育后期自手部存在相当大的调节潜力。
