Frayssinet P, Autefage A
Bioland, Toulouse.
Rev Rhum Ed Fr. 1993 May;60(5):342-51.
Hybrid materials for use as bone substitutes are composed of a synthetic substance to which autologous osteogenic cells are bound. Identification of osteogenic stem cells among bone marrow stromal cells was followed by the demonstration that a host of growth factors, hormones, and cytokines have effects on cultured osteoblasts. Porous ceramics have been developed as vectors for osteogenic cells previously grown in vitro. Porous hydroxyapatite beads bearing stromal cells were used to fill experimental ulnar defects in dogs. The cells were first grown on flat supports then on the three-dimensional hydroxyapatite network. Invasion by histologically identifiable bone tissue was seen in the stroma cell-bearing implants but not in control implants without stromal cells. Materials containing osteogenic cells may prove extremely useful since bone substitutes including hydroxyapatite ceramics are implanted in sites where osteogenic cells are scarce.
用作骨替代物的混合材料由结合了自体成骨细胞的合成物质组成。在骨髓基质细胞中鉴定出成骨干细胞之后，又证明了许多生长因子、激素和细胞因子对培养的成骨细胞有影响。多孔陶瓷已被开发为预先在体外培养的成骨细胞的载体。带有基质细胞的多孔羟基磷灰石珠被用于填充犬的实验性尺骨缺损。细胞首先在平坦的载体上生长，然后在三维羟基磷灰石网络上生长。在含有基质细胞的植入物中可见组织学上可识别的骨组织侵入，而在没有基质细胞的对照植入物中则未见。由于包括羟基磷灰石陶瓷在内的骨替代物被植入成骨细胞稀缺的部位，含有成骨细胞的材料可能会被证明极其有用。
