Uchida A, Nade S, McCartney E, Ching W
J Biomed Mater Res. 1987 Jan;21(1):1-10. doi: 10.1002/jbm.820210106.
Following the transplantation of bone marrow cells to extra-osseous sites, bone formation occurs in those sites. This osteogenic potential of bone marrow cells might be utilized for filling defects in bone if they could be transported on porous ceramic materials. Before such an approach becomes feasible, it is important to know what happens to the cells in the presence of the ceramics that might be used. In order to investigate the interaction between bone marrow cells and ceramics, in vitro, a system for culturing bone marrow cells on ceramic materials has been developed. Bone marrow cells adhered well to the surface of calcium hydroxyapatite and tricalcium phosphate ceramics, and this was followed by the formation of fibrous tissue on and within the ceramics. These ceramics were compatible with bone marrow cells even in culture conditions in which there was a large surface area of ceramic interfacing with cells. The results support the proposal that calcium hydroxyapatite and tricalcium phosphate are appropriate as bone replacement materials. In contrast, calcium aluminate had an adverse effect on bone marrow cells when there was a high proportion of ceramic to culture medium. However, this effect was not present if the proportion of ceramic to culture medium was low. Therefore, a large amount of biodegradable porous calcium aluminate ceramic should not be used as an alternative to autogeneous bone grafting.
将骨髓细胞移植到骨外部位后，这些部位会形成骨组织。如果骨髓细胞能够负载于多孔陶瓷材料上，其成骨潜能或许可用于填充骨缺损。在这种方法可行之前，了解骨髓细胞在可能使用的陶瓷材料存在的情况下会发生什么非常重要。为了研究骨髓细胞与陶瓷材料之间的相互作用，已开发出一种在体外将骨髓细胞培养于陶瓷材料上的系统。骨髓细胞能够很好地附着于羟基磷灰石和磷酸三钙陶瓷表面，随后在陶瓷材料表面及内部形成纤维组织。即使在陶瓷与细胞接触的表面积很大的培养条件下，这些陶瓷材料也与骨髓细胞具有良好的相容性。这些结果支持了羟基磷灰石和磷酸三钙作为骨替代材料是合适的这一观点。相比之下，当陶瓷与培养基的比例较高时，铝酸钙对骨髓细胞有不良影响。然而，如果陶瓷与培养基的比例较低，则不存在这种影响。因此，大量可生物降解的多孔铝酸钙陶瓷不应作为自体骨移植的替代品。
