Sleytr U B, Thorne K J
J Bacteriol. 1976 Apr;126(1):377-83. doi: 10.1128/jb.126.1.377-383.1976.
Clostridum thermosaccharolyticum and Clostridium thermohydrosulfuricum possess as outermost cell wall layer a tetragonal or hexagonal ordered array of macromolecules. The subunits of the surface layer can be detached from isolated cell walls with urea (8M) or guanidine-HCl (4 to 5 M). Triton X-100, dithiothreitol, ethylenediaminetetracetate, and KCl (3 M) had no visible effect on the regular arrays. Sodium dodecyl sulfate-polyacrylamide electrophroesis showed that, in both organisms, the surface layer is composed of glycoprotein of molecular weight 140,000. The glycoprotein from both microorganisms has a predominantly acidic amino acid composition and an acidic isoelectric point after isoelectric focusing on polyacrylamide gels. The glycocomponent is composed of glucose, galactose, mannose, and rhamnose.
嗜热解糖梭菌和嗜热硫化氢梭菌的最外层细胞壁层是由大分子构成的四方或六方有序阵列。表层亚基可用尿素（8M）或盐酸胍（4至5M）从分离的细胞壁上分离下来。Triton X - 100、二硫苏糖醇、乙二胺四乙酸和氯化钾（3M）对规则阵列没有明显影响。十二烷基硫酸钠 - 聚丙烯酰胺电泳表明，在这两种生物体中，表层均由分子量为140,000的糖蛋白组成。两种微生物的糖蛋白主要由酸性氨基酸组成，在聚丙烯酰胺凝胶上进行等电聚焦后具有酸性等电点。糖成分由葡萄糖、半乳糖、甘露糖和鼠李糖组成。
