Ogmundsdóttir H M, Pétursdóttir I, Gudmundsdóttir I, Amundadóttir L, Rønnov-Jessen L, Petersen O W
Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, Reykjavík.
In Vitro Cell Dev Biol Anim. 1993 Dec;29A(12):936-42. doi: 10.1007/BF02634232.
Breast carcinomas commonly contain varying amounts of fibrous stroma and infiltrates of lymphoid cells. Dickson and Lippman (Endocrine Rev., 8,29, 1987) have proposed a model of growth regulation in breast cancer involving interactions between stroma and carcinoma cells. This model is based on results obtained with established cell lines. In an effort to bring experimentation closer to the clinical situation we have used short-term primary cultures from human breast cancer in co-cultures with lymphocytes and fibroblasts. Cultures were established in a chemically defined serum-free medium (CDM3). Cell types were characterized on the basis of live morphology and expression of vimentin and keratin 18. A semi-quantitative system was developed for measuring growth of epithelial cells, thus defining two indices: maximal growth index (GI-max) and growth rate (GR). Moderate-to-good growth was obtained from 34 out of 46 carcinoma samples (74%) and 30 out of 38 parallel samples of non-cancerous tissue (79%). Success in culture was negatively correlated with the amount of hard stroma but unrelated to age of patient or clinical status. Malignant epithelium was clearly identified in 12 out of 34 (35%) carcinoma samples. For the evaluation of responses of epithelial cells in co-cultures, the cultures from each sample were ranked according to GI-max.(ABSTRACT TRUNCATED AT 250 WORDS)
乳腺癌通常含有不同数量的纤维性基质以及淋巴细胞浸润。迪克森和利普曼（《内分泌学评论》，第8卷，第29页，1987年）提出了一种乳腺癌生长调节模型，该模型涉及基质与癌细胞之间的相互作用。此模型基于用已建立的细胞系所获得的结果。为了使实验更贴近临床情况，我们使用了来自人类乳腺癌的短期原代培养物，将其与淋巴细胞和成纤维细胞进行共培养。培养物在化学成分明确的无血清培养基（CDM3）中建立。根据活细胞形态以及波形蛋白和角蛋白18的表达来对细胞类型进行表征。开发了一种半定量系统来测量上皮细胞的生长，从而定义了两个指标：最大生长指数（GI-max）和生长速率（GR）。46个癌样本中有34个（74%）以及38个平行的非癌组织样本中有30个（79%）获得了中度至良好的生长。培养成功与否与硬基质的量呈负相关，但与患者年龄或临床状况无关。在34个癌样本中有12个（35%）能清晰鉴定出恶性上皮。为了评估共培养中上皮细胞的反应，根据GI-max对每个样本的培养物进行排名。（摘要截选至250词）
