一种用于检测圆斑蝰蛇毒液的灵敏荧光酶联免疫吸附测定法。

A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom.

作者信息

Bhatti A R, Wong J P, Siddiqui Y M, Siddiqui S

机构信息

Defence Research Establishment Suffield, Department of National Defence, Medicine Hat, Alberta, Canada.

出版信息

Nat Toxins. 1993;1(5):277-82. doi: 10.1002/nt.2620010504.

DOI:10.1002/nt.2620010504

PMID:8167946

Abstract

A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA) has been adapted for the detection of Vipera russelli venom. The assay sensitivity was observed to be 0.1 pg/ml (1 x 10(-13) g/ml). Venoms from snakes of the Vipera group exhibited a high degree of cross-reactivity when tested with the antibody raised against V. russelli venom. With the exception of venom from Naja naja, all the tested venoms from unrelated families also showed cross-reactivity. This procedure is useful for detecting snake venom or its components in biological samples.

摘要

一种高灵敏度的荧光酶联免疫吸附测定法（FELISA）已被用于检测罗素蝰蛇毒液。该测定法的灵敏度为0.1 pg/ml（1×10⁻¹³ g/ml）。用针对罗素蝰蛇毒液产生的抗体进行检测时，蝰蛇属蛇类的毒液表现出高度的交叉反应性。除眼镜蛇毒液外，所有来自不相关科的受试毒液也显示出交叉反应性。该方法对于检测生物样品中的蛇毒或其成分很有用。