Nakamura S, Ueki T, Yao T, Ueyama T, Tsuneyoshi M
Second Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cancer. 1994 May 1;73(9):2239-49. doi: 10.1002/1097-0142(19940501)73:9<2239::aid-cncr2820730902>3.0.co;2-#.
Gastric carcinoma with lymphoid stroma (GCLS) has been reported to have a more favorable prognosis than ordinary gastric carcinoma, however, the precise mechanism of the pathogenesis for GCLS remains unclear.
The authors analyzed 99 GCLS in 94 patients for Epstein-Barr virus (EBV) sequences using polymerase chain reaction (PCR) and in situ hybridization (ISH); these were compared with 42 ordinary gastric carcinomas.
Two series of PCR showed 81 (82%) and 46 (47%) of the 99 GCLS to have EBV sequences, which were significantly higher compared with ordinary gastric carcinoma (50% and 9.5%, respectively). With ISH using thymine-thymine dimerized oligonucleotide probes corresponding to EBV-encoded small RNA 1 (EBER1), 82 (83%) of 99 GCLS showed clear, intense hybridization signals localized over the nuclei of the tumor cells, in contrast to only 4 (9.5%) of 42 ordinary carcinomas (P < 0.001). A comparative morphologic analysis of EBER1-positive and negative GCLS revealed that typical features of GCLS, such as mild cellular pleomorphism, rare mitoses, a marked degree of lymphoid stroma, and mild fibrosis, together with a lymphoid infiltration within the cancer cell nests were significantly more frequent in EBER1-positive GCLS.
More than 80% of GCLS were associated with EBV. The presence of EBV association in GCLS was characterized by the above morphologic features.
据报道，伴有淋巴样间质的胃癌（GCLS）比普通胃癌预后更好，然而，GCLS发病的确切机制仍不清楚。
作者使用聚合酶链反应（PCR）和原位杂交（ISH）分析了94例患者的99例GCLS中的爱泼斯坦-巴尔病毒（EBV）序列；并将其与42例普通胃癌进行比较。
两轮PCR显示，99例GCLS中有81例（82%）和46例（47%）含有EBV序列，这与普通胃癌相比显著更高（分别为50%和9.5%）。使用与EBV编码的小RNA 1（EBER1）对应的胸腺嘧啶-胸腺嘧啶二聚化寡核苷酸探针进行ISH检测，99例GCLS中有82例（83%）在肿瘤细胞核上显示清晰、强烈的杂交信号，相比之下，42例普通癌中只有4例（9.5%）出现这种情况（P<0.001）。对EBER1阳性和阴性GCLS进行的比较形态学分析显示，GCLS的典型特征，如轻度细胞多形性、罕见的有丝分裂、显著程度的淋巴样间质和轻度纤维化，以及癌细胞巢内的淋巴样浸润，在EBER1阳性GCLS中明显更常见。
超过80%的GCLS与EBV相关。GCLS中EBV相关性的存在具有上述形态学特征。
