Shibata D, Tokunaga M, Uemura Y, Sato E, Tanaka S, Weiss L M
Department of Pathology, Los Angeles County-University of Southern California Medical Center 90033.
Am J Pathol. 1991 Sep;139(3):469-74.
Some undifferentiated gastric carcinomas with intense lymphoid infiltration have a striking resemblance to nasopharyngeal lymphoepithelioma. The authors identified eight such cases (seven patients from Japan and one from the United States) of undifferentiated gastric carcinoma (lymphoepithelioma-like carcinoma [LELC]) and examined them for Epstein-Barr (EBV) viral sequences using polymerase chain reaction (PCR) and in situ hybridization (ISH) techniques. EBV was detected in seven of the eight cases by PCR, including a lymph-node metastasis. ISH that was performed in six of these cases showed EBV genomes to be uniformly present in the carcinoma cells and not present in the reactive lymphoid infiltrate or normal gastric mucosa. PCR of a polymorphic EBV locus (lymphocyte-determined membrane antigen) showed that a single genotype was present in each gastric LELC, consistent with a clonal process. These findings suggest that some undifferentiated gastric carcinomas are EBV-related and that focal EBV infection occurs before transformation.
一些伴有密集淋巴细胞浸润的未分化胃癌与鼻咽淋巴上皮瘤极为相似。作者鉴定出8例此类未分化胃癌（淋巴上皮瘤样癌[LELC]）病例（7例来自日本，1例来自美国），并使用聚合酶链反应（PCR）和原位杂交（ISH）技术检测其中的爱泼斯坦-巴尔（EBV）病毒序列。通过PCR在8例中的7例中检测到EBV，包括1例淋巴结转移。在其中6例中进行的ISH显示EBV基因组均匀存在于癌细胞中，而不存在于反应性淋巴细胞浸润或正常胃黏膜中。对一个多态性EBV位点（淋巴细胞决定膜抗原）进行的PCR显示，每个胃LELC中存在单一基因型，这与克隆过程一致。这些发现表明，一些未分化胃癌与EBV相关，并且在肿瘤转化之前发生了局灶性EBV感染。
