Picardo A L, Torres A J, Maestro M, Ortega D, Garcia-Asenjo J A, Mugüerza J M, Hernando F, Diez M, Balibrea J L
Department of General Surgery, II, Hospital Universitario de San Carlos, Madrid, Spain.
Cancer. 1994 May 1;73(9):2305-11. doi: 10.1002/1097-0142(19940501)73:9<2305::aid-cncr2820730911>3.0.co;2-d.
The cytosolic content of carcinoembryonic antigen (CEA), squamous cell carcinoma (SCC), CA 125, and CA 50 antigens in non-small cell lung cancer (NSCLC) is analyzed in this study. The aim was to ascertain the relationship between tumor marker content and the clinicopathologic aspects of this neoplasm.
Lung tissue samples were obtained at the time of surgery from 75 patients with NSCLC patients (samples of tumor and unaffected tissue) and 29 subjects with idiopathic pneumothorax. All determinations were performed on cytosols obtained from lung specimens. CEA and CA 125 were determined by enzyme immunoassay, SCC antigen by radioimmunoassay, and CA 50 by fluoroimmunoassay. Tumor marker content was analyzed by TNM stage, histologic type, tumor grade, and number of atypias.
The concentration of the four markers was significantly higher in cytosol obtained from neoplastic tissue. Frequency of elevated levels of CEA was higher in adenocarcinoma (87% cases expressing high levels of the marker), SCC antigen in epidermoid carcinoma (65% expressing high levels), and CA 125 in large cell carcinomas (100% expressing high levels). No association was found between TNM stage and cytosol concentration for any of the four markers. CEA exhibited significantly greater concentration in well differentiated tumors, whereas this was true of CA 125 in poorly differentiated tumors. CA 125 content was higher in tumors with more atypia.
Cytosolic quantification of tumor markers may be an adjuvant mechanism to evaluate histologic subtypes of non-small cell lung cancer and identification of tumors with poorly differentiated features.
本研究分析了非小细胞肺癌（NSCLC）中癌胚抗原（CEA）、鳞状细胞癌抗原（SCC）、CA 125和CA 50抗原的胞浆含量。目的是确定肿瘤标志物含量与该肿瘤临床病理特征之间的关系。
在手术时从75例NSCLC患者（肿瘤和未受影响组织的样本）和29例特发性气胸患者中获取肺组织样本。所有测定均在从肺标本中获得的胞浆上进行。CEA和CA 125通过酶免疫测定法测定，SCC抗原通过放射免疫测定法测定，CA 50通过荧光免疫测定法测定。通过TNM分期、组织学类型、肿瘤分级和异型性数量分析肿瘤标志物含量。
从肿瘤组织获得的胞浆中四种标志物的浓度显著更高。腺癌中CEA水平升高的频率更高（87%的病例表达高水平的标志物），表皮样癌中SCC抗原水平升高的频率更高（65%表达高水平），大细胞癌中CA 125水平升高的频率更高（100%表达高水平）。对于这四种标志物中的任何一种，均未发现TNM分期与胞浆浓度之间存在关联。CEA在高分化肿瘤中的浓度显著更高，而CA 125在低分化肿瘤中的情况则相反。CA 125含量在异型性更多的肿瘤中更高。
肿瘤标志物的胞浆定量可能是评估非小细胞肺癌组织学亚型和识别具有低分化特征肿瘤的辅助机制。
