Turner C A, Mack D H, Davis M M
Howard Hughes Medical Institute, Stanford University School of Medicine, California 94305-5428.
Cell. 1994 Apr 22;77(2):297-306. doi: 10.1016/0092-8674(94)90321-2.
We describe a novel gene, Blimp-1 (for B lymphocyte-induced maturation protein), transcripts of which are rapidly induced during the differentiation of B lymphocytes into immunoglobulin secretory cells and whose expression is characteristic of late B and plasma cell lines. The 856 amino acid open reading frame contains five Krüppel-type zinc finger motifs and proline-rich and acidic regions similar to those of known transcription factors. Serological studies show an approximately 100 kd protein that localizes to the nucleus. Stable or transient transfection of Blimp-1 into B cell lymphoma lines leads to the expression of many of the phenotypic changes associated with B cell differentiation into an early plasma cell stage, including induction of J chain message and immunoglobulin secretion, up-regulation of Syndecan-1, and increased cell size and granularity. Thus, Blimp-1 appears to be a pleiotropic regulatory factor capable of at least partially driving the terminal differentiation of B cells.
我们描述了一种新基因Blimp-1（B淋巴细胞诱导成熟蛋白），其转录本在B淋巴细胞分化为免疫球蛋白分泌细胞的过程中被迅速诱导，且其表达是晚期B细胞和浆细胞系的特征。这个856个氨基酸的开放阅读框包含五个Krüppel型锌指基序以及与已知转录因子相似的富含脯氨酸和酸性的区域。血清学研究显示一种定位在细胞核的约100kd的蛋白质。将Blimp-1稳定或瞬时转染到B细胞淋巴瘤系中会导致许多与B细胞分化为早期浆细胞阶段相关的表型变化的表达，包括J链信息的诱导和免疫球蛋白分泌、Syndecan-1的上调以及细胞大小和颗粒度的增加。因此，Blimp-1似乎是一种多效性调节因子，能够至少部分驱动B细胞的终末分化。
