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[绿茶提取物对单线态氧的清除作用及对脂质过氧化的预防作用]

[Scavenging action of green tea extracts on singlet oxygen and preventive effect on lipid peroxidation].

作者信息

Wu Y

机构信息

Institute of Basic Medical Sciences, CAMS, Beijing.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1993 Oct;15(5):354-9.

PMID:8168219
Abstract

Singlet oxygen (1O2) produced by illuminated photosensitizer Rose Bengal was detected by its bleaching effect on N,N-dimethylnitrosoaniline(DMNA). The 1O2 could be scavenged by green tea extracts (GTE), with the scavenging action being obvious at the early stage of photosensitized oxidation. The higher the concentration of GTE in the photooxidation reaction system, the stronger the 1O2 scavenging action. The scavenging effect of the fresh GTE solution on singlet oxygen was better than that of old solution. The preventive effects of GTE on lipid peroxidation induced by 1O2 in ghosts and microsomal membranes were also observed. Malondialdehyde (MDA) production was found to be completely inhibited by a high concentration of GTE in the reaction system.

摘要

通过光照光敏剂孟加拉玫瑰红产生的单线态氧(¹O₂),利用其对N,N - 二甲基亚硝基苯胺(DMNA)的漂白作用进行检测。¹O₂可被绿茶提取物(GTE)清除,在光敏氧化早期清除作用明显。光氧化反应体系中GTE浓度越高,¹O₂清除作用越强。新鲜GTE溶液对单线态氧的清除效果优于陈旧溶液。还观察到GTE对¹O₂诱导的红细胞膜和微粒体膜脂质过氧化的预防作用。发现反应体系中高浓度的GTE可完全抑制丙二醛(MDA)的产生。

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