Ostrowska Justyna, Łuczaj Wojciech, Kasacka Irena, Rózański Andrzej, Skrzydlewska Elzbieta
Department of Analytical Chemistry, Medical Academy of Bialystok, PO Box 14, 15-230 Bialystok, Poland.
Alcohol. 2004 Jan;32(1):25-32. doi: 10.1016/j.alcohol.2003.11.001.
Ethanol metabolism is accompanied by generation of free radicals, which stimulates lipid peroxidation. Natural antioxidants are particularly useful in such a situation. The current study was designed to investigate the efficacy of green tea, as a source of water-soluble antioxidants (catechins), on lipid peroxidation in liver, brain, and blood induced by chronic (4 weeks) ethanol intoxication in rats. Feeding of ethanol led to a significant increase in lipid peroxidation, as measured by increased concentrations of lipid hydroperoxides, 4-hydroxynonenal, and malondialdehyde. Feeding of ethanol also changed the glutathione-dependent lipid hydroperoxide decomposition system, resulting in a decrease in both reduced glutathione concentration and activity of glutathione peroxidase. Observed changes were statistically significant in all examined tissues. Enhancement in lipid peroxidation was associated with disruption of hepatocyte cell membranes, as observed through electron microscopic evaluation. Green tea protects phospholipids from enhanced peroxidation and prevents changes in biochemical parameters and morphologic changes observed after ethanol consumption. These results support the suggestion that green tea protects membranes from peroxidation of lipids associated with ethanol consumption.
乙醇代谢伴随着自由基的产生，这会刺激脂质过氧化。在这种情况下，天然抗氧化剂特别有用。本研究旨在探讨绿茶作为水溶性抗氧化剂（儿茶素）的来源，对大鼠慢性（4周）乙醇中毒诱导的肝脏、大脑和血液中脂质过氧化的影响。喂食乙醇导致脂质过氧化显著增加，通过脂质氢过氧化物、4-羟基壬烯醛和丙二醛浓度的增加来衡量。喂食乙醇还改变了谷胱甘肽依赖性脂质氢过氧化物分解系统，导致还原型谷胱甘肽浓度和谷胱甘肽过氧化物酶活性均降低。在所有检查的组织中，观察到的变化具有统计学意义。通过电子显微镜评估观察到，脂质过氧化的增强与肝细胞膜的破坏有关。绿茶可保护磷脂免受增强的过氧化作用，并防止乙醇摄入后观察到的生化参数变化和形态学变化。这些结果支持了绿茶可保护膜免受与乙醇摄入相关的脂质过氧化作用这一观点。
