Kang Y
PUMC Hospital, CAMS, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1993 Oct;15(5):380-4.
To investigate the effect of hyperinsulinemia on insulin action in target cells, insulin binding to adipocyte membranes and glucose oxidation in adipose cells were measured in control and experimentally hyperinsulinemic rats. The results showed that: 1) body weight, food intake and adipocyte diameter were not significantly changed, but serum insulin was significantly increased and blood glucose was markedly decreased in hyperinsulinemic rats as compared to control; 2) adipocyte membranes from hyperinsulinemic rats bound significantly less insulin than those from control rats, with a decreased number of insulin receptors and unchanged affinity of receptors for insulin; 3) significantly increased basal (in absence of added insulin) and insulin stimulated glucose oxidation was found in hyperinsulinemic rats as compared with control. The data suggest that experimental hyperinsulinemia resulted in decreased numbers of insulin receptors but increased responsiveness to insulin in target cells.
为研究高胰岛素血症对靶细胞胰岛素作用的影响，在对照大鼠和实验性高胰岛素血症大鼠中测定了胰岛素与脂肪细胞膜的结合以及脂肪细胞中的葡萄糖氧化。结果显示：1）与对照组相比，高胰岛素血症大鼠的体重、食物摄入量和脂肪细胞直径无显著变化，但血清胰岛素显著升高，血糖明显降低；2）高胰岛素血症大鼠的脂肪细胞膜与胰岛素的结合显著少于对照大鼠，胰岛素受体数量减少，受体对胰岛素的亲和力不变；3）与对照组相比，高胰岛素血症大鼠的基础（未添加胰岛素时）葡萄糖氧化和胰岛素刺激的葡萄糖氧化均显著增加。这些数据表明，实验性高胰岛素血症导致靶细胞中胰岛素受体数量减少，但对胰岛素的反应性增加。
