Kim J J, Quinn P A, Fortier M A
Department of Ontogeny and Reproduction, Centre Hospitalier de l'Université Laval, Ste. Foy, Quebec.
Infect Immun. 1994 May;62(5):1528-33. doi: 10.1128/iai.62.5.1528-1533.1994.
Bovine epithelial and stromal cells of the endometrium were inoculated with Ureaplasma diversum, pathogenic strain 2312, at 10(6) or 10(3) color-changing units (ccu)/ml in the presence of 1% fetal bovine serum (depleted of steroids by dextran-charcoal treatment) to assess the effect of infection on prostaglandin biosynthesis. When the inoculum of U. diversum was 10(6) ccu/ml, the concentration of U. diversum in the culture medium decreased with time. U. diversum was found on the epithelial and stromal cell monolayers, increasing in titer 100-fold, indicating that attachment and eventually growth occurred. When the inoculum was 10(3) ccu/ml, the titer of U. diversum remained the same or increased in the supernatant and increased on epithelial and stromal cells. The effect of infection was evaluated by measurement of the primary prostaglandin produced by each cell type, prostaglandin F2a for epithelial cells and prostaglandin E2 for stromal cells. Infection with U. diversum significantly decreased prostaglandin F2a accumulation, by 44.7% +/- 6.0% at 10(6) ccu/ml (P < or = 0.005) and 15.8% +/- 5.3% at 10(3) ccu/ml (P < or = 0.05) in epithelial cells. Prostaglandin E2 accumulation by stromal cells was decreased by 34.0% +/- 4.0% at 10(6) ccu/ml (P < or = 0.001) and by 13.5% +/- 2.7% at 10(3) ccu/ml (P < or = 0.005). Infection with 10(6) ccu/ml did not alter endometrial cell viability, as shown by protein measurement, trypan blue dye exclusion, and cell plating efficiency tests. Thus, alterations in prostaglandin production were not due to cell deterioration. These observations suggest that U. diversum can alter prostaglandin E2 and prostaglandin F2a patterns in primary cultures of bovine endometrial cells without affecting cell viability.
在含有1%胎牛血清（经葡聚糖 - 活性炭处理去除类固醇）的条件下，将子宫内膜的牛上皮细胞和基质细胞接种10(6)或10(3)个颜色变化单位（ccu）/毫升的差异脲原体致病菌株2312，以评估感染对前列腺素生物合成的影响。当差异脲原体接种量为10(6) ccu/毫升时，培养基中差异脲原体的浓度随时间下降。在单层上皮细胞和基质细胞上发现了差异脲原体，其滴度增加了100倍，表明发生了附着并最终生长。当接种量为10(3) ccu/毫升时，差异脲原体在上清液中的滴度保持不变或增加，并且在上皮细胞和基质细胞上也增加。通过测量每种细胞类型产生的主要前列腺素（上皮细胞产生前列腺素F2α，基质细胞产生前列腺素E2）来评估感染的影响。差异脲原体感染显著降低了前列腺素F2α的积累，在10(6) ccu/毫升时降低了44.7%±6.0%（P≤0.005），在10(3) ccu/毫升时降低了15.8%±5.3%（P≤0.05），在上皮细胞中。基质细胞中前列腺素E2的积累在10(6) ccu/毫升时降低了34.0%±4.0%（P≤0.001），在10(3) ccu/毫升时降低了13.5%±2.7%（P≤0.005）。如蛋白质测量、台盼蓝染料排斥试验和细胞接种效率测试所示，接种10(6) ccu/毫升并未改变子宫内膜细胞的活力。因此，前列腺素产生的改变并非由于细胞恶化。这些观察结果表明，差异脲原体可改变牛子宫内膜细胞原代培养中前列腺素E2和前列腺素F2α的模式，而不影响细胞活力。
