用于检测T株支原体抗体的颜色试验。
Color test for the measurement of antibody to T-strain mycoplasmas.
作者信息
Purcell R H, Taylor-Robinson D, Wong D, Chanock R M
出版信息
J Bacteriol. 1966 Jul;92(1):6-12. doi: 10.1128/jb.92.1.6-12.1966.
Abstract
Purcell, Robert H. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), D. Taylor-Robinson, D. Wong, and R. M. Chanock. Color test for the measurement of antibody to T-strain mycoplasmas. J. Bacteriol. 92:6-12. 1966.-A metabolic inhibition technique for the measurement of antibody to T-strain mycoplasmas was developed, based upon the ability of T-strain mycoplasmas to metabolize urea with the concomitant production of ammonia, and the ability of specific antiserum to inhibit this ammonia production. Phenol red added to the medium served as an indicator of pH change resulting from ammonia production. Specific antiserum to T-strain mycoplasma T-960 was prepared. The T-strain organism was shown to be serologically distinct from the recognized large-colony mycoplasmas. Antibody to mycoplasma strain T-960 in human sera was demonstrated with the metabolic inhibition technique.
摘要
珀塞尔，罗伯特·H.（国立过敏和传染病研究所，马里兰州贝塞斯达），D. 泰勒 - 罗宾逊，D. 黄，以及R. M. 查诺克。用于检测T株支原体抗体的颜色试验。《细菌学杂志》92:6 - 12。1966年。——基于T株支原体代谢尿素并伴随产生氨的能力以及特异性抗血清抑制这种氨产生的能力，开发了一种用于检测T株支原体抗体的代谢抑制技术。添加到培养基中的酚红用作氨产生导致的pH变化的指示剂。制备了针对T株支原体T - 960的特异性抗血清。已证明T株生物体在血清学上与公认的大菌落支原体不同。用代谢抑制技术在人血清中检测到了针对支原体菌株T - 960的抗体。
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