Chantler J K, Lund K D, Miki N P, Berkowitz C A, Tai G
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
Intervirology. 1993;36(4):225-36. doi: 10.1159/000150341.
A comparison of the phenotypic properties of three rubella vaccines (HPV77/DE5, RA27/3 and Cendehill) and four wild-type (wt+) isolates (M33, Therien, Thomas and IB2) has been carried out. Differences in growth characteristics, plaque morphology and temperature sensitivity were identified. In addition differential reactivity of the strains to polyclonal and a monoclonal anti-E1 antibody were found in immunoperoxidase-staining reactions. The ability of the wt+ and vaccine strains to infect lymphoreticular cells and chondrocytes, also varied in that the RA27/3 and Cendehill strains were highly restricted in both these cell types while the wt+ strains and HPV77/DE5 vaccine grew to higher titer. This biological variation was associated with differences in E1 and E2 glycoproteins detected on immunoblots.
对三种风疹疫苗（HPV77/DE5、RA27/3和Cendehill）和四种野生型（wt+）分离株（M33、Therien、Thomas和IB2）的表型特性进行了比较。确定了生长特性、蚀斑形态和温度敏感性方面的差异。此外，在免疫过氧化物酶染色反应中发现这些菌株对多克隆和单克隆抗E1抗体的反应性存在差异。wt+菌株和疫苗株感染淋巴网状细胞和软骨细胞的能力也有所不同，RA27/3和Cendehill菌株在这两种细胞类型中受到高度限制，而wt+菌株和HPV77/DE5疫苗则生长到更高滴度。这种生物学差异与免疫印迹上检测到的E1和E2糖蛋白差异有关。
